Colonic lesions, cytokine profiles, and gut microbiota in plasminogen-deficient mice

Bill Vestergaard; Lukasz Krych; Leif R. Lund; Bettina Merete Pyndt Jørgensen; Lars H. Hansen; Henrik Elvang Jensen; Dennis Sandris Nielsen; Axel Kornerup Hansen

Colonic lesions, cytokine profiles, and gut microbiota in plasminogen-deficient mice

Bill Vestergaard, Lukasz Krych, Leif R. Lund, Bettina Merete Pyndt Jørgensen, Lars H. Hansen, Henrik Elvang Jensen, Dennis Sandris Nielsen, Axel Kornerup Hansen

Abstract

Plasminogen-deficient (FVB/NPan-plg(tm1Jld), plg(tm1Jld)) mice, which are widely used as a wound-healing model, are prone to spontaneous rectal prolapses. The aims of this study were 1) to evaluate the fecal microbiome of plg(tm1Jld) mice for features that might contribute to the development of rectal prolapses and colonic inflammation and 2) to assess the relevance of the inflammatory phenotype to the variability in wound healing in this model. The (plgtm1Jld) mice exhibited delayed wound healing, and they could be divided into 3 distinct groups that differed according to the time until wound closure. Colonic lesions in plg(tm1Jld) mice, which were characterized by necrotizing ulcerations and cystically dilated glands, were restricted to the intermediate and distal parts of the colon. The cytokine profile was indicative of chronic tissue damage, but the genetic modification did not change the composition of the gut microbiota, and none of the clinical or biochemical parameters correlated with the gut microbiota composition.

Original language	English
Journal	Comparative Medicine
Volume	65
Issue number	5
Pages (from-to)	382-397
Number of pages	16
ISSN	1532-0820
Publication status	Published - Oct 2015

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title = "Colonic lesions, cytokine profiles, and gut microbiota in plasminogen-deficient mice",

abstract = "Plasminogen-deficient (FVB/NPan-plg(tm1Jld), plg(tm1Jld)) mice, which are widely used as a wound-healing model, are prone to spontaneous rectal prolapses. The aims of this study were 1) to evaluate the fecal microbiome of plg(tm1Jld) mice for features that might contribute to the development of rectal prolapses and colonic inflammation and 2) to assess the relevance of the inflammatory phenotype to the variability in wound healing in this model. The (plgtm1Jld) mice exhibited delayed wound healing, and they could be divided into 3 distinct groups that differed according to the time until wound closure. Colonic lesions in plg(tm1Jld) mice, which were characterized by necrotizing ulcerations and cystically dilated glands, were restricted to the intermediate and distal parts of the colon. The cytokine profile was indicative of chronic tissue damage, but the genetic modification did not change the composition of the gut microbiota, and none of the clinical or biochemical parameters correlated with the gut microbiota composition.",

author = "Bill Vestergaard and Lukasz Krych and Lund, {Leif R.} and J{\o}rgensen, {Bettina Merete Pyndt} and Hansen, {Lars H.} and Jensen, {Henrik Elvang} and Nielsen, {Dennis Sandris} and Hansen, {Axel Kornerup}",

year = "2015",

month = oct,

language = "English",

volume = "65",

pages = "382--397",

journal = "Comparative Medicine",

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T1 - Colonic lesions, cytokine profiles, and gut microbiota in plasminogen-deficient mice

AU - Vestergaard, Bill

AU - Krych, Lukasz

AU - Lund, Leif R.

AU - Jørgensen, Bettina Merete Pyndt

AU - Hansen, Lars H.

AU - Jensen, Henrik Elvang

AU - Nielsen, Dennis Sandris

AU - Hansen, Axel Kornerup

PY - 2015/10

Y1 - 2015/10

N2 - Plasminogen-deficient (FVB/NPan-plg(tm1Jld), plg(tm1Jld)) mice, which are widely used as a wound-healing model, are prone to spontaneous rectal prolapses. The aims of this study were 1) to evaluate the fecal microbiome of plg(tm1Jld) mice for features that might contribute to the development of rectal prolapses and colonic inflammation and 2) to assess the relevance of the inflammatory phenotype to the variability in wound healing in this model. The (plgtm1Jld) mice exhibited delayed wound healing, and they could be divided into 3 distinct groups that differed according to the time until wound closure. Colonic lesions in plg(tm1Jld) mice, which were characterized by necrotizing ulcerations and cystically dilated glands, were restricted to the intermediate and distal parts of the colon. The cytokine profile was indicative of chronic tissue damage, but the genetic modification did not change the composition of the gut microbiota, and none of the clinical or biochemical parameters correlated with the gut microbiota composition.

AB - Plasminogen-deficient (FVB/NPan-plg(tm1Jld), plg(tm1Jld)) mice, which are widely used as a wound-healing model, are prone to spontaneous rectal prolapses. The aims of this study were 1) to evaluate the fecal microbiome of plg(tm1Jld) mice for features that might contribute to the development of rectal prolapses and colonic inflammation and 2) to assess the relevance of the inflammatory phenotype to the variability in wound healing in this model. The (plgtm1Jld) mice exhibited delayed wound healing, and they could be divided into 3 distinct groups that differed according to the time until wound closure. Colonic lesions in plg(tm1Jld) mice, which were characterized by necrotizing ulcerations and cystically dilated glands, were restricted to the intermediate and distal parts of the colon. The cytokine profile was indicative of chronic tissue damage, but the genetic modification did not change the composition of the gut microbiota, and none of the clinical or biochemical parameters correlated with the gut microbiota composition.

M3 - Journal article

C2 - 26473342

SN - 1532-0820

VL - 65

SP - 382

EP - 397

JO - Comparative Medicine

JF - Comparative Medicine

IS - 5

ER -

Colonic lesions, cytokine profiles, and gut microbiota in plasminogen-deficient mice

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