Collection of apoplastic fluids from Arabidopsis thaliana leaves

Svend Roesen Madsen; Hussam Hassan Nour-Eldin; Barbara Ann Halkier

doi:10.1007/978-1-4939-3393-8_4

Collection of apoplastic fluids from Arabidopsis thaliana leaves

Svend Roesen Madsen, Hussam Hassan Nour-Eldin, Barbara Ann Halkier

Section for Molecular Plant Biology

5 Citations (Scopus)

Abstract

The leaf apoplast comprises the extracellular continuum outside cell membranes. A broad range of processes take place in the apoplast, including intercellular signaling, metabolite transport, and plant-microbe interactions. To study these processes, it is essential to analyze the metabolite content in apoplastic fluids. Due to the fragile nature of leaf tissues, it is a challenge to obtain apoplastic fluids from leaves. Here, methods to collect apoplastic washing fluid and guttation fluid from Arabidopsis thaliana leaves are described.

Original language	English
Title of host publication	Biotechnology of plant secondary metabolism : methods and protocols
Number of pages	8
Publisher	Springer
Publication date	2016
Pages	35-42
Chapter	4
ISBN (Print)	978-1-4939-3391-4
ISBN (Electronic)	978-1-4939-3393-8
DOIs	https://doi.org/10.1007/978-1-4939-3393-8_4
Publication status	Published - 2016

Series	Methods in Molecular Biology
Volume	1405
ISSN	1064-3745

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Collection of apoplastic fluids from Arabidopsis thaliana leaves. / Madsen, Svend Roesen; Nour-Eldin, Hussam Hassan ; Halkier, Barbara Ann.
Biotechnology of plant secondary metabolism: methods and protocols. Springer, 2016. p. 35-42 (Methods in Molecular Biology, Vol. 1405).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

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Collection of apoplastic fluids from Arabidopsis thaliana leaves

Abstract

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