TY - CHAP
T1 - Collection of apoplastic fluids from Arabidopsis thaliana leaves
AU - Madsen, Svend Roesen
AU - Nour-Eldin, Hussam Hassan
AU - Halkier, Barbara Ann
PY - 2016
Y1 - 2016
N2 - The leaf apoplast comprises the extracellular continuum outside cell membranes. A broad range of processes take place in the apoplast, including intercellular signaling, metabolite transport, and plant-microbe interactions. To study these processes, it is essential to analyze the metabolite content in apoplastic fluids. Due to the fragile nature of leaf tissues, it is a challenge to obtain apoplastic fluids from leaves. Here, methods to collect apoplastic washing fluid and guttation fluid from Arabidopsis thaliana leaves are described.
AB - The leaf apoplast comprises the extracellular continuum outside cell membranes. A broad range of processes take place in the apoplast, including intercellular signaling, metabolite transport, and plant-microbe interactions. To study these processes, it is essential to analyze the metabolite content in apoplastic fluids. Due to the fragile nature of leaf tissues, it is a challenge to obtain apoplastic fluids from leaves. Here, methods to collect apoplastic washing fluid and guttation fluid from Arabidopsis thaliana leaves are described.
U2 - 10.1007/978-1-4939-3393-8_4
DO - 10.1007/978-1-4939-3393-8_4
M3 - Book chapter
C2 - 26843163
SN - 978-1-4939-3391-4
T3 - Methods in Molecular Biology
SP - 35
EP - 42
BT - Biotechnology of plant secondary metabolism
PB - Springer
ER -