Abstract
Cobalamin and its binding protein, haptocorrin, are present in rat milk throughout the lactation period. The concentration of cobalamin is approximately 0.3-times the concentration of the unsaturated binding protein. The concentration of the unsaturated cobalamin-binding protein varies between 18 nmol l-1 and 16 nmol l-1. The binding protein has a Stokes radius of 2.49 nm when saturated with cobalamin and 2.61 nm when unsaturated. It binds cobalamin over a broad range of pH and is able to bind cobinamide also. With immunohistochemistry, we find haptocorrin immunoreactivity in the mammary glands and in the sebaceous glands of the nipple.
| Original language | English |
|---|---|
| Journal | Scandinavian Journal of Clinical & Laboratory Investigation |
| Volume | 49 |
| Issue number | 6 |
| Pages (from-to) | 529-35 |
| Number of pages | 7 |
| ISSN | 0036-5513 |
| Publication status | Published - Oct 1989 |
Keywords
- Animals
- Chromatography, Gel
- Female
- Immunohistochemistry
- Lactation
- Mammary Glands, Animal
- Milk
- Pregnancy
- Rats
- Rats, Inbred Strains
- Transcobalamins
- Vitamin B 12