ClpP-dependent and -independent activities encoded by the polycistronic clpK-encoding locus contribute to heat shock survival in Klebsiella pneumoniae

Martin Saxtorph Bojer; Carsten Struve; Hanne Ingmer; Karen Angeliki Krogfelt

doi:10.1016/j.resmic.2012.11.005

ClpP-dependent and -independent activities encoded by the polycistronic clpK-encoding locus contribute to heat shock survival in Klebsiella pneumoniae

Martin Saxtorph Bojer, Carsten Struve, Hanne Ingmer, Karen Angeliki Krogfelt

11 Citations (Scopus)

Abstract

The family of Clp ATPases plays an important role in bacterial physiology. Here we characterize the genetic locus encompassing a newly described plasmid-encoded ClpK protein protecting Klebsiella pneumoniae cells during heat shock. We demonstrate that the clpK gene is located in a polycistronic operon and that the variable downstream gene content correlates with heat-resistant phenotypes of different isolates. ClpK is encoded by a multifunctional transcriptional unit characterized by both ClpP-dependent and -independent activities. Notably, our data show that ClpP is indispensible for thermoprotection exerted by ClpK alone, suggesting that ClpK is a new member of the family of ClpP-interacting Clp ATPases.

Original language	English
Journal	Research in Microbiology
Volume	164
Issue number	3
Pages (from-to)	205-210
Number of pages	6
ISSN	0923-2508
DOIs	https://doi.org/10.1016/j.resmic.2012.11.005
Publication status	Published - Apr 2013
Externally published	Yes

Keywords

Former LIFE faculty

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10.1016/j.resmic.2012.11.005

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ClpP-dependent and -independent activities encoded by the polycistronic clpK-encoding locus contribute to heat shock survival in Klebsiella pneumoniae. / Bojer, Martin Saxtorph; Struve, Carsten; Ingmer, Hanne et al.

In: Research in Microbiology, Vol. 164, No. 3, 04.2013, p. 205-210.

Research output: Contribution to journal › Journal article › Research › peer-review

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title = "ClpP-dependent and -independent activities encoded by the polycistronic clpK-encoding locus contribute to heat shock survival in Klebsiella pneumoniae",

abstract = "The family of Clp ATPases plays an important role in bacterial physiology. Here we characterize the genetic locus encompassing a newly described plasmid-encoded ClpK protein protecting Klebsiella pneumoniae cells during heat shock. We demonstrate that the clpK gene is located in a polycistronic operon and that the variable downstream gene content correlates with heat-resistant phenotypes of different isolates. ClpK is encoded by a multifunctional transcriptional unit characterized by both ClpP-dependent and -independent activities. Notably, our data show that ClpP is indispensible for thermoprotection exerted by ClpK alone, suggesting that ClpK is a new member of the family of ClpP-interacting Clp ATPases.",

keywords = "Former LIFE faculty, Klebsiella pneumoniae, Clp ATPase, ClpK, Heat resistance, ClpP, sHSP",

author = "Bojer, {Martin Saxtorph} and Carsten Struve and Hanne Ingmer and Krogfelt, {Karen Angeliki}",

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T1 - ClpP-dependent and -independent activities encoded by the polycistronic clpK-encoding locus contribute to heat shock survival in Klebsiella pneumoniae

AU - Bojer, Martin Saxtorph

AU - Struve, Carsten

AU - Ingmer, Hanne

AU - Krogfelt, Karen Angeliki

PY - 2013/4

Y1 - 2013/4

N2 - The family of Clp ATPases plays an important role in bacterial physiology. Here we characterize the genetic locus encompassing a newly described plasmid-encoded ClpK protein protecting Klebsiella pneumoniae cells during heat shock. We demonstrate that the clpK gene is located in a polycistronic operon and that the variable downstream gene content correlates with heat-resistant phenotypes of different isolates. ClpK is encoded by a multifunctional transcriptional unit characterized by both ClpP-dependent and -independent activities. Notably, our data show that ClpP is indispensible for thermoprotection exerted by ClpK alone, suggesting that ClpK is a new member of the family of ClpP-interacting Clp ATPases.

AB - The family of Clp ATPases plays an important role in bacterial physiology. Here we characterize the genetic locus encompassing a newly described plasmid-encoded ClpK protein protecting Klebsiella pneumoniae cells during heat shock. We demonstrate that the clpK gene is located in a polycistronic operon and that the variable downstream gene content correlates with heat-resistant phenotypes of different isolates. ClpK is encoded by a multifunctional transcriptional unit characterized by both ClpP-dependent and -independent activities. Notably, our data show that ClpP is indispensible for thermoprotection exerted by ClpK alone, suggesting that ClpK is a new member of the family of ClpP-interacting Clp ATPases.

KW - Former LIFE faculty

KW - Klebsiella pneumoniae

KW - Clp ATPase

KW - ClpK

KW - Heat resistance

KW - ClpP

KW - sHSP

U2 - 10.1016/j.resmic.2012.11.005

DO - 10.1016/j.resmic.2012.11.005

M3 - Journal article

C2 - 23201206

SN - 0923-2508

VL - 164

SP - 205

EP - 210

JO - Research in Microbiology

JF - Research in Microbiology

IS - 3

ER -

ClpP-dependent and -independent activities encoded by the polycistronic clpK-encoding locus contribute to heat shock survival in Klebsiella pneumoniae

Abstract

Keywords

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