ClpP-dependent and -independent activities encoded by the polycistronic clpK-encoding locus contribute to heat shock survival in Klebsiella pneumoniae

Martin Saxtorph Bojer; Carsten Struve; Hanne Ingmer; Karen Angeliki Krogfelt

doi:10.1016/j.resmic.2012.11.005

ClpP-dependent and -independent activities encoded by the polycistronic clpK-encoding locus contribute to heat shock survival in Klebsiella pneumoniae

Martin Saxtorph Bojer, Carsten Struve, Hanne Ingmer, Karen Angeliki Krogfelt

11 Citationer (Scopus)

Abstract

The family of Clp ATPases plays an important role in bacterial physiology. Here we characterize the genetic locus encompassing a newly described plasmid-encoded ClpK protein protecting Klebsiella pneumoniae cells during heat shock. We demonstrate that the clpK gene is located in a polycistronic operon and that the variable downstream gene content correlates with heat-resistant phenotypes of different isolates. ClpK is encoded by a multifunctional transcriptional unit characterized by both ClpP-dependent and -independent activities. Notably, our data show that ClpP is indispensible for thermoprotection exerted by ClpK alone, suggesting that ClpK is a new member of the family of ClpP-interacting Clp ATPases.

Originalsprog	Engelsk
Tidsskrift	Research in Microbiology
Vol/bind	164
Udgave nummer	3
Sider (fra-til)	205-210
Antal sider	6
ISSN	0923-2508
DOI	https://doi.org/10.1016/j.resmic.2012.11.005
Status	Udgivet - apr. 2013
Udgivet eksternt	Ja

Emneord

Det tidligere LIFE
Klebsiella pneumoniae
Clp ATPase
ClpK
Heat resistance
ClpP
sHSP

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10.1016/j.resmic.2012.11.005

Citationsformater

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title = "ClpP-dependent and -independent activities encoded by the polycistronic clpK-encoding locus contribute to heat shock survival in Klebsiella pneumoniae",

abstract = "The family of Clp ATPases plays an important role in bacterial physiology. Here we characterize the genetic locus encompassing a newly described plasmid-encoded ClpK protein protecting Klebsiella pneumoniae cells during heat shock. We demonstrate that the clpK gene is located in a polycistronic operon and that the variable downstream gene content correlates with heat-resistant phenotypes of different isolates. ClpK is encoded by a multifunctional transcriptional unit characterized by both ClpP-dependent and -independent activities. Notably, our data show that ClpP is indispensible for thermoprotection exerted by ClpK alone, suggesting that ClpK is a new member of the family of ClpP-interacting Clp ATPases.",

keywords = "Former LIFE faculty, Klebsiella pneumoniae, Clp ATPase, ClpK, Heat resistance, ClpP, sHSP",

author = "Bojer, {Martin Saxtorph} and Carsten Struve and Hanne Ingmer and Krogfelt, {Karen Angeliki}",

year = "2013",

month = apr,

doi = "10.1016/j.resmic.2012.11.005",

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volume = "164",

pages = "205--210",

journal = "Research in Microbiology",

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TY - JOUR

T1 - ClpP-dependent and -independent activities encoded by the polycistronic clpK-encoding locus contribute to heat shock survival in Klebsiella pneumoniae

AU - Bojer, Martin Saxtorph

AU - Struve, Carsten

AU - Ingmer, Hanne

AU - Krogfelt, Karen Angeliki

PY - 2013/4

Y1 - 2013/4

N2 - The family of Clp ATPases plays an important role in bacterial physiology. Here we characterize the genetic locus encompassing a newly described plasmid-encoded ClpK protein protecting Klebsiella pneumoniae cells during heat shock. We demonstrate that the clpK gene is located in a polycistronic operon and that the variable downstream gene content correlates with heat-resistant phenotypes of different isolates. ClpK is encoded by a multifunctional transcriptional unit characterized by both ClpP-dependent and -independent activities. Notably, our data show that ClpP is indispensible for thermoprotection exerted by ClpK alone, suggesting that ClpK is a new member of the family of ClpP-interacting Clp ATPases.

AB - The family of Clp ATPases plays an important role in bacterial physiology. Here we characterize the genetic locus encompassing a newly described plasmid-encoded ClpK protein protecting Klebsiella pneumoniae cells during heat shock. We demonstrate that the clpK gene is located in a polycistronic operon and that the variable downstream gene content correlates with heat-resistant phenotypes of different isolates. ClpK is encoded by a multifunctional transcriptional unit characterized by both ClpP-dependent and -independent activities. Notably, our data show that ClpP is indispensible for thermoprotection exerted by ClpK alone, suggesting that ClpK is a new member of the family of ClpP-interacting Clp ATPases.

KW - Former LIFE faculty

KW - Klebsiella pneumoniae

KW - Clp ATPase

KW - ClpK

KW - Heat resistance

KW - ClpP

KW - sHSP

U2 - 10.1016/j.resmic.2012.11.005

DO - 10.1016/j.resmic.2012.11.005

M3 - Journal article

C2 - 23201206

SN - 0923-2508

VL - 164

SP - 205

EP - 210

JO - Research in Microbiology

JF - Research in Microbiology

IS - 3

ER -

ClpP-dependent and -independent activities encoded by the polycistronic clpK-encoding locus contribute to heat shock survival in Klebsiella pneumoniae

Abstract

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