Cloning of the pig aminopeptidase N gene. Identification of possible regulatory elements and the exon distribution in relation to the membrane-spanning region.

H Sjöström, O Norén, Jørgen Olsen

36 Citations (Scopus)

Abstract

We have isolated four lambda-phages covering the complete pig aminopeptidase N/CD13 gene. The sequence of 2.85 kbp encompasses 1.18 kbp of the 5' upstream region and 1.67 kbp of the structural gene. In the promoter region we find a TATA box and potential binding sites for CTF-1/NF-1 and AP-2. By sequence comparisons we have found three domains showing similarity to promoter regions of the genes encoding human alpha 1-antitrypsin and human intestinal alkaline phosphatase. The gene sequence includes the first three exons and two introns. It shows that a single exon encodes the cytoplasmic tail, the membrane anchor and the junctional peptide.
Original languageEnglish
JournalFEBS Letters
Volume251
Issue number1-2
Pages (from-to)275-81
Number of pages6
ISSN0014-5793
Publication statusPublished - 1989

Fingerprint

Dive into the research topics of 'Cloning of the pig aminopeptidase N gene. Identification of possible regulatory elements and the exon distribution in relation to the membrane-spanning region.'. Together they form a unique fingerprint.

Cite this