TY - JOUR
T1 - Cloning and characterization of the adipokinetic hormone receptor from the cockroach Periplaneta americana.
AU - Hansen, Karina K
AU - Hauser, Frank
AU - Cazzamali, Giuseppe
AU - Williamson, Michael
AU - Grimmelikhuijzen, Cornelis J P
N1 - Keywords: Amino Acid Sequence; Animals; Cloning, Molecular; Conserved Sequence; Evolution, Molecular; Insect Hormones; Molecular Sequence Data; Oligopeptides; Periplaneta; Pyrrolidonecarboxylic Acid; Receptors, G-Protein-Coupled; Receptors, Neuropeptide; Sequence Homology, Amino Acid; Species Specificity
PY - 2006
Y1 - 2006
N2 - Cockroaches have long been used as insect models to investigate the actions of biologically active neuropeptides. Here, we describe the cloning and functional expression in Chinese hamster ovary cells of an adipokinetic hormone (AKH) G protein-coupled receptor from the cockroach Periplaneta americana. This receptor is only activated by various insect AKHs (we tested eight) and not by a library of 29 other insect or invertebrate neuropeptides and nine biogenic amines. Periplaneta has two intrinsic AKHs, Pea-AKH-1, and Pea-AKH-2. The Periplaneta AKH receptor is activated by low concentrations of both Pea-AKH-1 (EC50, 5 x 10(-9)M), and Pea-AKH-2 (EC50, 2 x 10(-9)M). Insects can be subdivided into two evolutionary lineages, holometabola (insects with a complete metamorphosis during development) and hemimetabola (incomplete metamorphosis). This paper describes the first AKH receptor from a hemimetabolous insect.
AB - Cockroaches have long been used as insect models to investigate the actions of biologically active neuropeptides. Here, we describe the cloning and functional expression in Chinese hamster ovary cells of an adipokinetic hormone (AKH) G protein-coupled receptor from the cockroach Periplaneta americana. This receptor is only activated by various insect AKHs (we tested eight) and not by a library of 29 other insect or invertebrate neuropeptides and nine biogenic amines. Periplaneta has two intrinsic AKHs, Pea-AKH-1, and Pea-AKH-2. The Periplaneta AKH receptor is activated by low concentrations of both Pea-AKH-1 (EC50, 5 x 10(-9)M), and Pea-AKH-2 (EC50, 2 x 10(-9)M). Insects can be subdivided into two evolutionary lineages, holometabola (insects with a complete metamorphosis during development) and hemimetabola (incomplete metamorphosis). This paper describes the first AKH receptor from a hemimetabolous insect.
U2 - 10.1016/j.bbrc.2006.03.012
DO - 10.1016/j.bbrc.2006.03.012
M3 - Journal article
C2 - 16554038
SN - 0006-291X
VL - 343
SP - 638
EP - 643
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -