Circulating levels of platelet α-granule cytokines in trauma patients

TY - JOUR

T1 - Circulating levels of platelet α-granule cytokines in trauma patients

AU - Windeløv, Nis Agerlin

AU - Ostrowski, Sisse Rye

AU - Johansson, Per Ingemar

AU - Wanscher, Michael Christopher J

AU - Larsen, Claus Falck

AU - Sørensen, A M

AU - Rasmussen, Lars S.

PY - 2015/3/13

Y1 - 2015/3/13

N2 - Objective and design: To elucidate whether platelets differentiate cytokine release following trauma, we prospectively measured three major platelet-derived cytokines in 213 trauma patients on hospital arrival.Methods: We measured plasma levels of the anti-inflammatory β-thromboglobulins (βTGs), transforming growth factor-β1 (TGFβ1) and the pro-inflammatory platelet factor 4 (PF4) cytokines. We also measured soluble glycoprotein VI (sGPVI), procoagulant platelet microparticles (PMPs) and white blood cell (WBC) counts, and evaluated in vitro platelet function in primary and secondary haemostasis by aggregometry and thromboelastometry, respectively. We evaluated associations of each cytokine by multivariate regression including injury severity score (ISS), WBC counts, sGPVI and platelet counts as explanatory variables.Results: Severely injured patients (ISS > 15) had higher levels of βTGs and TGFβ1 (both p < 0.01) but lower levels of PF4 (p = 0.02). GPVI and PMPs levels correlated with TGFβ1 and PF4 whereas we found no significant association between cytokine levels and measures of haemostasis. By multivariate regression, a high WBC count was associated with high levels of TGFβ1 (p = 0.01) and βTGs (p < 0.01) but with low levels of PF4 (p = 0.03).Conclusion: Severely injured patients had higher levels of βTGs and TGFβ1 but lower levels of the PF4; a high WBC count predicted this anti-inflammatory profile of platelet cytokines.

AB - Objective and design: To elucidate whether platelets differentiate cytokine release following trauma, we prospectively measured three major platelet-derived cytokines in 213 trauma patients on hospital arrival.Methods: We measured plasma levels of the anti-inflammatory β-thromboglobulins (βTGs), transforming growth factor-β1 (TGFβ1) and the pro-inflammatory platelet factor 4 (PF4) cytokines. We also measured soluble glycoprotein VI (sGPVI), procoagulant platelet microparticles (PMPs) and white blood cell (WBC) counts, and evaluated in vitro platelet function in primary and secondary haemostasis by aggregometry and thromboelastometry, respectively. We evaluated associations of each cytokine by multivariate regression including injury severity score (ISS), WBC counts, sGPVI and platelet counts as explanatory variables.Results: Severely injured patients (ISS > 15) had higher levels of βTGs and TGFβ1 (both p < 0.01) but lower levels of PF4 (p = 0.02). GPVI and PMPs levels correlated with TGFβ1 and PF4 whereas we found no significant association between cytokine levels and measures of haemostasis. By multivariate regression, a high WBC count was associated with high levels of TGFβ1 (p = 0.01) and βTGs (p < 0.01) but with low levels of PF4 (p = 0.03).Conclusion: Severely injured patients had higher levels of βTGs and TGFβ1 but lower levels of the PF4; a high WBC count predicted this anti-inflammatory profile of platelet cytokines.

KW - Adult

KW - Female

KW - Humans

KW - Leukocyte Count

KW - Male

KW - Middle Aged

KW - Platelet Factor 4

KW - Platelet Membrane Glycoproteins

KW - Regression Analysis

KW - Retrospective Studies

KW - Severity of Illness Index

KW - Transforming Growth Factor beta1

KW - Wounds and Injuries

KW - beta-Thromboglobulin

U2 - 10.1007/s00011-015-0802-4

DO - 10.1007/s00011-015-0802-4

M3 - Journal article

C2 - 25697747

SN - 1023-3830

VL - 64

SP - 235

EP - 241

JO - Inflammation Research

JF - Inflammation Research

IS - 3-4

ER -