TY - JOUR
T1 - Circulating levels of platelet α-granule cytokines in trauma patients
AU - Windeløv, Nis Agerlin
AU - Ostrowski, Sisse Rye
AU - Johansson, Per Ingemar
AU - Wanscher, Michael Christopher J
AU - Larsen, Claus Falck
AU - Sørensen, A M
AU - Rasmussen, Lars S.
PY - 2015/3/13
Y1 - 2015/3/13
N2 - Objective and design: To elucidate whether platelets differentiate cytokine release following trauma, we prospectively measured three major platelet-derived cytokines in 213 trauma patients on hospital arrival.Methods: We measured plasma levels of the anti-inflammatory β-thromboglobulins (βTGs), transforming growth factor-β1 (TGFβ1) and the pro-inflammatory platelet factor 4 (PF4) cytokines. We also measured soluble glycoprotein VI (sGPVI), procoagulant platelet microparticles (PMPs) and white blood cell (WBC) counts, and evaluated in vitro platelet function in primary and secondary haemostasis by aggregometry and thromboelastometry, respectively. We evaluated associations of each cytokine by multivariate regression including injury severity score (ISS), WBC counts, sGPVI and platelet counts as explanatory variables.Results: Severely injured patients (ISS > 15) had higher levels of βTGs and TGFβ1 (both p < 0.01) but lower levels of PF4 (p = 0.02). GPVI and PMPs levels correlated with TGFβ1 and PF4 whereas we found no significant association between cytokine levels and measures of haemostasis. By multivariate regression, a high WBC count was associated with high levels of TGFβ1 (p = 0.01) and βTGs (p < 0.01) but with low levels of PF4 (p = 0.03).Conclusion: Severely injured patients had higher levels of βTGs and TGFβ1 but lower levels of the PF4; a high WBC count predicted this anti-inflammatory profile of platelet cytokines.
AB - Objective and design: To elucidate whether platelets differentiate cytokine release following trauma, we prospectively measured three major platelet-derived cytokines in 213 trauma patients on hospital arrival.Methods: We measured plasma levels of the anti-inflammatory β-thromboglobulins (βTGs), transforming growth factor-β1 (TGFβ1) and the pro-inflammatory platelet factor 4 (PF4) cytokines. We also measured soluble glycoprotein VI (sGPVI), procoagulant platelet microparticles (PMPs) and white blood cell (WBC) counts, and evaluated in vitro platelet function in primary and secondary haemostasis by aggregometry and thromboelastometry, respectively. We evaluated associations of each cytokine by multivariate regression including injury severity score (ISS), WBC counts, sGPVI and platelet counts as explanatory variables.Results: Severely injured patients (ISS > 15) had higher levels of βTGs and TGFβ1 (both p < 0.01) but lower levels of PF4 (p = 0.02). GPVI and PMPs levels correlated with TGFβ1 and PF4 whereas we found no significant association between cytokine levels and measures of haemostasis. By multivariate regression, a high WBC count was associated with high levels of TGFβ1 (p = 0.01) and βTGs (p < 0.01) but with low levels of PF4 (p = 0.03).Conclusion: Severely injured patients had higher levels of βTGs and TGFβ1 but lower levels of the PF4; a high WBC count predicted this anti-inflammatory profile of platelet cytokines.
KW - Adult
KW - Female
KW - Humans
KW - Leukocyte Count
KW - Male
KW - Middle Aged
KW - Platelet Factor 4
KW - Platelet Membrane Glycoproteins
KW - Regression Analysis
KW - Retrospective Studies
KW - Severity of Illness Index
KW - Transforming Growth Factor beta1
KW - Wounds and Injuries
KW - beta-Thromboglobulin
U2 - 10.1007/s00011-015-0802-4
DO - 10.1007/s00011-015-0802-4
M3 - Journal article
C2 - 25697747
SN - 1023-3830
VL - 64
SP - 235
EP - 241
JO - Inflammation Research
JF - Inflammation Research
IS - 3-4
ER -