Circulating levels of microRNA from children with newly diagnosed type 1 diabetes and healthy controls: evidence that miR-25 associates to residual beta-cell function and glycaemic control during disease progression

Lotte B Nielsen; Cheng Wang; Kaspar Sørensen; Claus H Bang-Berthelsen; Lars Hansen; Marie Louise Max Andersen; Philip Hougaard; Anders Juul; Chen-Yu Zhang; Flemming Pociot; Henrik B Mortensen

doi:10.1155/2012/896362

Circulating levels of microRNA from children with newly diagnosed type 1 diabetes and healthy controls: evidence that miR-25 associates to residual beta-cell function and glycaemic control during disease progression

Lotte B Nielsen, Cheng Wang, Kaspar Sørensen, Claus H Bang-Berthelsen, Lars Hansen, Marie Louise Max Andersen, Philip Hougaard, Anders Juul, Chen-Yu Zhang, Flemming Pociot, Henrik B Mortensen

161 Citations (Scopus)

Abstract

This study aims to identify key miRNAs in circulation, which predict ongoing beta-cell destruction and regeneration in children with newly diagnosed Type 1 Diabetes (T1D). We compared expression level of sera miRNAs from new onset T1D children and age-matched healthy controls and related the miRNAs expression levels to beta-cell function and glycaemic control. Global miRNA sequencing analyses were performed on sera pools from two T1D cohorts (n = 275 and 129, resp.) and one control group (n = 151). We identified twelve upregulated human miRNAs in T1D patients (miR-152, miR-30a-5p, miR-181a, miR-24, miR-148a, miR-210, miR-27a, miR-29a, miR-26a, miR-27b, miR-25, miR-200a); several of these miRNAs were linked to apoptosis and beta-cell networks. Furthermore, we identified miR-25 as negatively associated with residual beta-cell function (est.: -0.12, P = 0.0037), and positively associated with glycaemic control (HbA1c) (est.: 0.11, P = 0.0035) 3 months after onset. In conclusion this study demonstrates that miR-25 might be a tissue-specific miRNA for glycaemic control 3 months after diagnosis in new onset T1D children and therefore supports the role of circulating miRNAs as predictive biomarkers for tissue physiopathology and potential intervention targets.

Original language	English
Journal	Experimental Diabetes Research
Volume	2012
Pages (from-to)	896362
Number of pages	7
ISSN	1687-5214
DOIs	https://doi.org/10.1155/2012/896362
Publication status	Published - 2012

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Nielsen, L. B., Wang, C., Sørensen, K., Bang-Berthelsen, C. H., Hansen, L., Andersen, M. L. M., Hougaard, P., Juul, A., Zhang, C-Y., Pociot, F., & Mortensen, H. B. (2012). Circulating levels of microRNA from children with newly diagnosed type 1 diabetes and healthy controls: evidence that miR-25 associates to residual beta-cell function and glycaemic control during disease progression. Experimental Diabetes Research, 2012, 896362. https://doi.org/10.1155/2012/896362

Circulating levels of microRNA from children with newly diagnosed type 1 diabetes and healthy controls : evidence that miR-25 associates to residual beta-cell function and glycaemic control during disease progression. / Nielsen, Lotte B; Wang, Cheng; Sørensen, Kaspar et al.

In: Experimental Diabetes Research, Vol. 2012, 2012, p. 896362.

Research output: Contribution to journal › Journal article › Research › peer-review

Nielsen, LB, Wang, C, Sørensen, K, Bang-Berthelsen, CH, Hansen, L, Andersen, MLM, Hougaard, P, Juul, A, Zhang, C-Y, Pociot, F & Mortensen, HB 2012, 'Circulating levels of microRNA from children with newly diagnosed type 1 diabetes and healthy controls: evidence that miR-25 associates to residual beta-cell function and glycaemic control during disease progression', Experimental Diabetes Research, vol. 2012, pp. 896362. https://doi.org/10.1155/2012/896362

Nielsen LB, Wang C, Sørensen K, Bang-Berthelsen CH, Hansen L, Andersen MLM et al. Circulating levels of microRNA from children with newly diagnosed type 1 diabetes and healthy controls: evidence that miR-25 associates to residual beta-cell function and glycaemic control during disease progression. Experimental Diabetes Research. 2012;2012:896362. doi: 10.1155/2012/896362

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title = "Circulating levels of microRNA from children with newly diagnosed type 1 diabetes and healthy controls: evidence that miR-25 associates to residual beta-cell function and glycaemic control during disease progression",

abstract = "This study aims to identify key miRNAs in circulation, which predict ongoing beta-cell destruction and regeneration in children with newly diagnosed Type 1 Diabetes (T1D). We compared expression level of sera miRNAs from new onset T1D children and age-matched healthy controls and related the miRNAs expression levels to beta-cell function and glycaemic control. Global miRNA sequencing analyses were performed on sera pools from two T1D cohorts (n = 275 and 129, resp.) and one control group (n = 151). We identified twelve upregulated human miRNAs in T1D patients (miR-152, miR-30a-5p, miR-181a, miR-24, miR-148a, miR-210, miR-27a, miR-29a, miR-26a, miR-27b, miR-25, miR-200a); several of these miRNAs were linked to apoptosis and beta-cell networks. Furthermore, we identified miR-25 as negatively associated with residual beta-cell function (est.: -0.12, P = 0.0037), and positively associated with glycaemic control (HbA1c) (est.: 0.11, P = 0.0035) 3 months after onset. In conclusion this study demonstrates that miR-25 might be a tissue-specific miRNA for glycaemic control 3 months after diagnosis in new onset T1D children and therefore supports the role of circulating miRNAs as predictive biomarkers for tissue physiopathology and potential intervention targets.",

author = "Nielsen, {Lotte B} and Cheng Wang and Kaspar S{\o}rensen and Bang-Berthelsen, {Claus H} and Lars Hansen and Andersen, {Marie Louise Max} and Philip Hougaard and Anders Juul and Chen-Yu Zhang and Flemming Pociot and Mortensen, {Henrik B}",

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T2 - evidence that miR-25 associates to residual beta-cell function and glycaemic control during disease progression

AU - Nielsen, Lotte B

AU - Wang, Cheng

AU - Sørensen, Kaspar

AU - Bang-Berthelsen, Claus H

AU - Hansen, Lars

AU - Andersen, Marie Louise Max

AU - Hougaard, Philip

AU - Juul, Anders

AU - Zhang, Chen-Yu

AU - Pociot, Flemming

AU - Mortensen, Henrik B

PY - 2012

Y1 - 2012

N2 - This study aims to identify key miRNAs in circulation, which predict ongoing beta-cell destruction and regeneration in children with newly diagnosed Type 1 Diabetes (T1D). We compared expression level of sera miRNAs from new onset T1D children and age-matched healthy controls and related the miRNAs expression levels to beta-cell function and glycaemic control. Global miRNA sequencing analyses were performed on sera pools from two T1D cohorts (n = 275 and 129, resp.) and one control group (n = 151). We identified twelve upregulated human miRNAs in T1D patients (miR-152, miR-30a-5p, miR-181a, miR-24, miR-148a, miR-210, miR-27a, miR-29a, miR-26a, miR-27b, miR-25, miR-200a); several of these miRNAs were linked to apoptosis and beta-cell networks. Furthermore, we identified miR-25 as negatively associated with residual beta-cell function (est.: -0.12, P = 0.0037), and positively associated with glycaemic control (HbA1c) (est.: 0.11, P = 0.0035) 3 months after onset. In conclusion this study demonstrates that miR-25 might be a tissue-specific miRNA for glycaemic control 3 months after diagnosis in new onset T1D children and therefore supports the role of circulating miRNAs as predictive biomarkers for tissue physiopathology and potential intervention targets.

AB - This study aims to identify key miRNAs in circulation, which predict ongoing beta-cell destruction and regeneration in children with newly diagnosed Type 1 Diabetes (T1D). We compared expression level of sera miRNAs from new onset T1D children and age-matched healthy controls and related the miRNAs expression levels to beta-cell function and glycaemic control. Global miRNA sequencing analyses were performed on sera pools from two T1D cohorts (n = 275 and 129, resp.) and one control group (n = 151). We identified twelve upregulated human miRNAs in T1D patients (miR-152, miR-30a-5p, miR-181a, miR-24, miR-148a, miR-210, miR-27a, miR-29a, miR-26a, miR-27b, miR-25, miR-200a); several of these miRNAs were linked to apoptosis and beta-cell networks. Furthermore, we identified miR-25 as negatively associated with residual beta-cell function (est.: -0.12, P = 0.0037), and positively associated with glycaemic control (HbA1c) (est.: 0.11, P = 0.0035) 3 months after onset. In conclusion this study demonstrates that miR-25 might be a tissue-specific miRNA for glycaemic control 3 months after diagnosis in new onset T1D children and therefore supports the role of circulating miRNAs as predictive biomarkers for tissue physiopathology and potential intervention targets.

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DO - 10.1155/2012/896362

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