Circulating levels of microRNA from children with newly diagnosed type 1 diabetes and healthy controls: evidence that miR-25 associates to residual beta-cell function and glycaemic control during disease progression

Lotte B Nielsen; Cheng Wang; Kaspar Sørensen; Claus H Bang-Berthelsen; Lars Hansen; Marie Louise Max Andersen; Philip Hougaard; Anders Juul; Chen-Yu Zhang; Flemming Pociot; Henrik B Mortensen

doi:10.1155/2012/896362

Circulating levels of microRNA from children with newly diagnosed type 1 diabetes and healthy controls: evidence that miR-25 associates to residual beta-cell function and glycaemic control during disease progression

Lotte B Nielsen, Cheng Wang, Kaspar Sørensen, Claus H Bang-Berthelsen, Lars Hansen, Marie Louise Max Andersen, Philip Hougaard, Anders Juul, Chen-Yu Zhang, Flemming Pociot, Henrik B Mortensen

161 Citationer (Scopus)

Abstract

This study aims to identify key miRNAs in circulation, which predict ongoing beta-cell destruction and regeneration in children with newly diagnosed Type 1 Diabetes (T1D). We compared expression level of sera miRNAs from new onset T1D children and age-matched healthy controls and related the miRNAs expression levels to beta-cell function and glycaemic control. Global miRNA sequencing analyses were performed on sera pools from two T1D cohorts (n = 275 and 129, resp.) and one control group (n = 151). We identified twelve upregulated human miRNAs in T1D patients (miR-152, miR-30a-5p, miR-181a, miR-24, miR-148a, miR-210, miR-27a, miR-29a, miR-26a, miR-27b, miR-25, miR-200a); several of these miRNAs were linked to apoptosis and beta-cell networks. Furthermore, we identified miR-25 as negatively associated with residual beta-cell function (est.: -0.12, P = 0.0037), and positively associated with glycaemic control (HbA1c) (est.: 0.11, P = 0.0035) 3 months after onset. In conclusion this study demonstrates that miR-25 might be a tissue-specific miRNA for glycaemic control 3 months after diagnosis in new onset T1D children and therefore supports the role of circulating miRNAs as predictive biomarkers for tissue physiopathology and potential intervention targets.

Originalsprog	Engelsk
Tidsskrift	Experimental Diabetes Research
Vol/bind	2012
Sider (fra-til)	896362
Antal sider	7
ISSN	1687-5214
DOI	https://doi.org/10.1155/2012/896362
Status	Udgivet - 2012

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10.1155/2012/896362

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Citationsformater

Nielsen, L. B., Wang, C., Sørensen, K., Bang-Berthelsen, C. H., Hansen, L., Andersen, M. L. M., Hougaard, P., Juul, A., Zhang, C-Y., Pociot, F., & Mortensen, H. B. (2012). Circulating levels of microRNA from children with newly diagnosed type 1 diabetes and healthy controls: evidence that miR-25 associates to residual beta-cell function and glycaemic control during disease progression. Experimental Diabetes Research, 2012, 896362. https://doi.org/10.1155/2012/896362

Circulating levels of microRNA from children with newly diagnosed type 1 diabetes and healthy controls : evidence that miR-25 associates to residual beta-cell function and glycaemic control during disease progression. / Nielsen, Lotte B; Wang, Cheng; Sørensen, Kaspar et al.

I: Experimental Diabetes Research, Bind 2012, 2012, s. 896362.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Nielsen, LB, Wang, C, Sørensen, K, Bang-Berthelsen, CH, Hansen, L, Andersen, MLM, Hougaard, P, Juul, A, Zhang, C-Y, Pociot, F & Mortensen, HB 2012, 'Circulating levels of microRNA from children with newly diagnosed type 1 diabetes and healthy controls: evidence that miR-25 associates to residual beta-cell function and glycaemic control during disease progression', Experimental Diabetes Research, bind 2012, s. 896362. https://doi.org/10.1155/2012/896362

Nielsen LB, Wang C, Sørensen K, Bang-Berthelsen CH, Hansen L, Andersen MLM et al. Circulating levels of microRNA from children with newly diagnosed type 1 diabetes and healthy controls: evidence that miR-25 associates to residual beta-cell function and glycaemic control during disease progression. Experimental Diabetes Research. 2012;2012:896362. doi: 10.1155/2012/896362

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title = "Circulating levels of microRNA from children with newly diagnosed type 1 diabetes and healthy controls: evidence that miR-25 associates to residual beta-cell function and glycaemic control during disease progression",

abstract = "This study aims to identify key miRNAs in circulation, which predict ongoing beta-cell destruction and regeneration in children with newly diagnosed Type 1 Diabetes (T1D). We compared expression level of sera miRNAs from new onset T1D children and age-matched healthy controls and related the miRNAs expression levels to beta-cell function and glycaemic control. Global miRNA sequencing analyses were performed on sera pools from two T1D cohorts (n = 275 and 129, resp.) and one control group (n = 151). We identified twelve upregulated human miRNAs in T1D patients (miR-152, miR-30a-5p, miR-181a, miR-24, miR-148a, miR-210, miR-27a, miR-29a, miR-26a, miR-27b, miR-25, miR-200a); several of these miRNAs were linked to apoptosis and beta-cell networks. Furthermore, we identified miR-25 as negatively associated with residual beta-cell function (est.: -0.12, P = 0.0037), and positively associated with glycaemic control (HbA1c) (est.: 0.11, P = 0.0035) 3 months after onset. In conclusion this study demonstrates that miR-25 might be a tissue-specific miRNA for glycaemic control 3 months after diagnosis in new onset T1D children and therefore supports the role of circulating miRNAs as predictive biomarkers for tissue physiopathology and potential intervention targets.",

author = "Nielsen, {Lotte B} and Cheng Wang and Kaspar S{\o}rensen and Bang-Berthelsen, {Claus H} and Lars Hansen and Andersen, {Marie Louise Max} and Philip Hougaard and Anders Juul and Chen-Yu Zhang and Flemming Pociot and Mortensen, {Henrik B}",

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doi = "10.1155/2012/896362",

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T1 - Circulating levels of microRNA from children with newly diagnosed type 1 diabetes and healthy controls

T2 - evidence that miR-25 associates to residual beta-cell function and glycaemic control during disease progression

AU - Nielsen, Lotte B

AU - Wang, Cheng

AU - Sørensen, Kaspar

AU - Bang-Berthelsen, Claus H

AU - Hansen, Lars

AU - Andersen, Marie Louise Max

AU - Hougaard, Philip

AU - Juul, Anders

AU - Zhang, Chen-Yu

AU - Pociot, Flemming

AU - Mortensen, Henrik B

PY - 2012

Y1 - 2012

N2 - This study aims to identify key miRNAs in circulation, which predict ongoing beta-cell destruction and regeneration in children with newly diagnosed Type 1 Diabetes (T1D). We compared expression level of sera miRNAs from new onset T1D children and age-matched healthy controls and related the miRNAs expression levels to beta-cell function and glycaemic control. Global miRNA sequencing analyses were performed on sera pools from two T1D cohorts (n = 275 and 129, resp.) and one control group (n = 151). We identified twelve upregulated human miRNAs in T1D patients (miR-152, miR-30a-5p, miR-181a, miR-24, miR-148a, miR-210, miR-27a, miR-29a, miR-26a, miR-27b, miR-25, miR-200a); several of these miRNAs were linked to apoptosis and beta-cell networks. Furthermore, we identified miR-25 as negatively associated with residual beta-cell function (est.: -0.12, P = 0.0037), and positively associated with glycaemic control (HbA1c) (est.: 0.11, P = 0.0035) 3 months after onset. In conclusion this study demonstrates that miR-25 might be a tissue-specific miRNA for glycaemic control 3 months after diagnosis in new onset T1D children and therefore supports the role of circulating miRNAs as predictive biomarkers for tissue physiopathology and potential intervention targets.

AB - This study aims to identify key miRNAs in circulation, which predict ongoing beta-cell destruction and regeneration in children with newly diagnosed Type 1 Diabetes (T1D). We compared expression level of sera miRNAs from new onset T1D children and age-matched healthy controls and related the miRNAs expression levels to beta-cell function and glycaemic control. Global miRNA sequencing analyses were performed on sera pools from two T1D cohorts (n = 275 and 129, resp.) and one control group (n = 151). We identified twelve upregulated human miRNAs in T1D patients (miR-152, miR-30a-5p, miR-181a, miR-24, miR-148a, miR-210, miR-27a, miR-29a, miR-26a, miR-27b, miR-25, miR-200a); several of these miRNAs were linked to apoptosis and beta-cell networks. Furthermore, we identified miR-25 as negatively associated with residual beta-cell function (est.: -0.12, P = 0.0037), and positively associated with glycaemic control (HbA1c) (est.: 0.11, P = 0.0035) 3 months after onset. In conclusion this study demonstrates that miR-25 might be a tissue-specific miRNA for glycaemic control 3 months after diagnosis in new onset T1D children and therefore supports the role of circulating miRNAs as predictive biomarkers for tissue physiopathology and potential intervention targets.

U2 - 10.1155/2012/896362

DO - 10.1155/2012/896362

M3 - Journal article

C2 - 22829805

SN - 2314-6745

VL - 2012

SP - 896362

JO - Journal of Diabetes Research

JF - Journal of Diabetes Research

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