Chitin and stress induced protein kinase activation

Chandra Shekar Kenchappa; Raquel Azevedo da Silva; Simon Bressendorff; Sabrina Stanimirovic; Jakob Valdbjørn Olsen; Morten Petersen; John Mundy

doi:10.1007/978-1-4939-6859-6_15

Chitin and stress induced protein kinase activation

Chandra Shekar Kenchappa, Raquel Azevedo da Silva, Simon Bressendorff, Sabrina Stanimirovic, Jakob Valdbjørn Olsen, Morten Petersen, John Mundy^*

^*Corresponding author for this work

Functional Genomics

Abstract

The assays described here are pertinent to protein kinase studies in any plant. They include an immunoblot phosphorylation/activation assay and an in-gel activity assay for MAP kinases (MPKs) using the general protein kinase substrate myelin basic protein. They also include a novel in-gel peptide substrate assay for Snf1-related kinase family 2 members (SnRK2s). This kinase family-specific assay overcomes some limitations of in-gel assays and permits the identification of different types of kinase activities in total protein extracts.

Original language	English
Title of host publication	Plant pattern recognition receptors : methods and protocols
Editors	Libo Shan, Ping He
Number of pages	10
Publisher	Springer
Publication date	2017
Pages	185-194
Chapter	15
ISBN (Print)	978-1-4939-6858-9
ISBN (Electronic)	978-1-4939-6859-6
DOIs	https://doi.org/10.1007/978-1-4939-6859-6_15
Publication status	Published - 2017

Series	Methods in Molecular Biology
Volume	1578
ISSN	1064-3745

Keywords

Acryloylated peptide substrates
In-gel assays
Myelin basic protein

Access to Document

10.1007/978-1-4939-6859-6_15

Cite this

Kenchappa, C. S., Azevedo da Silva, R., Bressendorff, S., Stanimirovic, S., Olsen, J. V., Petersen, M., & Mundy, J. (2017). Chitin and stress induced protein kinase activation. In L. Shan, & P. He (Eds.), Plant pattern recognition receptors: methods and protocols (pp. 185-194). Springer. Methods in Molecular Biology Vol. 1578 https://doi.org/10.1007/978-1-4939-6859-6_15

Chitin and stress induced protein kinase activation. / Kenchappa, Chandra Shekar; Azevedo da Silva, Raquel; Bressendorff, Simon et al.

Plant pattern recognition receptors: methods and protocols. ed. / Libo Shan; Ping He. Springer, 2017. p. 185-194 (Methods in Molecular Biology, Vol. 1578).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

@inbook{87466919347b4decb6b9f175f474df86,

title = "Chitin and stress induced protein kinase activation",

abstract = "The assays described here are pertinent to protein kinase studies in any plant. They include an immunoblot phosphorylation/activation assay and an in-gel activity assay for MAP kinases (MPKs) using the general protein kinase substrate myelin basic protein. They also include a novel in-gel peptide substrate assay for Snf1-related kinase family 2 members (SnRK2s). This kinase family-specific assay overcomes some limitations of in-gel assays and permits the identification of different types of kinase activities in total protein extracts.",

keywords = "Acryloylated peptide substrates, In-gel assays, Myelin basic protein",

author = "Kenchappa, {Chandra Shekar} and {Azevedo da Silva}, Raquel and Simon Bressendorff and Sabrina Stanimirovic and Olsen, {Jakob Valdbj{\o}rn} and Morten Petersen and John Mundy",

year = "2017",

doi = "10.1007/978-1-4939-6859-6_15",

language = "English",

isbn = "978-1-4939-6858-9",

series = "Methods in Molecular Biology",

publisher = "Springer",

pages = "185--194",

editor = "Libo Shan and Ping He",

booktitle = "Plant pattern recognition receptors",

}

TY - CHAP

T1 - Chitin and stress induced protein kinase activation

AU - Kenchappa, Chandra Shekar

AU - Azevedo da Silva, Raquel

AU - Bressendorff, Simon

AU - Stanimirovic, Sabrina

AU - Olsen, Jakob Valdbjørn

AU - Petersen, Morten

AU - Mundy, John

PY - 2017

Y1 - 2017

N2 - The assays described here are pertinent to protein kinase studies in any plant. They include an immunoblot phosphorylation/activation assay and an in-gel activity assay for MAP kinases (MPKs) using the general protein kinase substrate myelin basic protein. They also include a novel in-gel peptide substrate assay for Snf1-related kinase family 2 members (SnRK2s). This kinase family-specific assay overcomes some limitations of in-gel assays and permits the identification of different types of kinase activities in total protein extracts.

AB - The assays described here are pertinent to protein kinase studies in any plant. They include an immunoblot phosphorylation/activation assay and an in-gel activity assay for MAP kinases (MPKs) using the general protein kinase substrate myelin basic protein. They also include a novel in-gel peptide substrate assay for Snf1-related kinase family 2 members (SnRK2s). This kinase family-specific assay overcomes some limitations of in-gel assays and permits the identification of different types of kinase activities in total protein extracts.

KW - Acryloylated peptide substrates

KW - In-gel assays

KW - Myelin basic protein

UR - http://www.scopus.com/inward/record.url?scp=85013647028&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-6859-6_15

DO - 10.1007/978-1-4939-6859-6_15

M3 - Book chapter

C2 - 28220425

AN - SCOPUS:85013647028

SN - 978-1-4939-6858-9

T3 - Methods in Molecular Biology

SP - 185

EP - 194

BT - Plant pattern recognition receptors

A2 - Shan, Libo

A2 - He, Ping

PB - Springer

ER -

Chitin and stress induced protein kinase activation

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this