@inbook{87466919347b4decb6b9f175f474df86,
title = "Chitin and stress induced protein kinase activation",
abstract = "The assays described here are pertinent to protein kinase studies in any plant. They include an immunoblot phosphorylation/activation assay and an in-gel activity assay for MAP kinases (MPKs) using the general protein kinase substrate myelin basic protein. They also include a novel in-gel peptide substrate assay for Snf1-related kinase family 2 members (SnRK2s). This kinase family-specific assay overcomes some limitations of in-gel assays and permits the identification of different types of kinase activities in total protein extracts.",
keywords = "Acryloylated peptide substrates, In-gel assays, Myelin basic protein",
author = "Kenchappa, {Chandra Shekar} and {Azevedo da Silva}, Raquel and Simon Bressendorff and Sabrina Stanimirovic and Olsen, {Jakob Valdbj{\o}rn} and Morten Petersen and John Mundy",
year = "2017",
doi = "10.1007/978-1-4939-6859-6_15",
language = "English",
isbn = "978-1-4939-6858-9",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "185--194",
editor = "Libo Shan and Ping He",
booktitle = "Plant pattern recognition receptors",
}