Chemo-enzymatic production of O-glycopeptides for the detection of serum glycopeptide antibodies

Alexander Nøstdal, Hans H Wandall

3 Citations (Scopus)

Abstract

Protein microarray is a highly sensitive tool for antibody detection in serum. Monitoring of patients' antibody titers to specific antigens is increasingly employed in the diagnosis of several conditions, ranging from infectious diseases, allergies, autoimmune diseases, and cancer. In this protocol we present a detailed method for enzymatic generation of disease-specific O-glycopeptides and how to monitor the antibody response to these in serum using microarray technology.

Original languageEnglish
Title of host publication Immunoproteomics : Methods in Molecular Biology
EditorsKelly M. Fulton, Susan M. Twine
Number of pages13
Volume1061
PublisherHumana Press
Publication date2013
Pages167-79
Chapter10
ISBN (Print)978-1-62703-589-7
ISBN (Electronic)978-1-62703-589-7
DOIs
Publication statusPublished - 2013
SeriesMethods in molecular biology (Clifton, N.J.)

Keywords

  • Animals
  • Antibodies
  • Enzymes
  • Glycopeptides
  • Humans
  • Protein Array Analysis

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