Chemo-enzymatic production of O-glycopeptides for the detection of serum glycopeptide antibodies

Alexander Nøstdal; Hans H Wandall

doi:10.1007/978-1-62703-589-7_10

Chemo-enzymatic production of O-glycopeptides for the detection of serum glycopeptide antibodies

Alexander Nøstdal, Hans H Wandall

Glycomics Program

3 Citationer (Scopus)

Abstract

Protein microarray is a highly sensitive tool for antibody detection in serum. Monitoring of patients’ antibody titers to specifi c antigens is increasingly employed in the diagnosis of several conditions, ranging from infectious diseases, allergies, autoimmune diseases, and cancer. In this protocol we present a detailed
method for enzymatic generation of disease-specifi c O- glycopeptides and how to monitor the antibody response to these in serum using microarray technology.

Originalsprog	Engelsk
Titel	Immunoproteomics : Methods in Molecular Biology
Redaktører	Kelly M. Fulton, Susan M. Twine
Antal sider	13
Vol/bind	1061
Forlag	Humana Press
Publikationsdato	2013
Sider	167-79
Kapitel	10
ISBN (Trykt)	978-1-62703-589-7
ISBN (Elektronisk)	978-1-62703-589-7
DOI	https://doi.org/10.1007/978-1-62703-589-7_10
Status	Udgivet - 2013

Navn	Methods in molecular biology (Clifton, N.J.)

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10.1007/978-1-62703-589-7_10

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Chemo-enzymatic production of O-glycopeptides for the detection of serum glycopeptide antibodies. / Nøstdal, Alexander; Wandall, Hans H.

Immunoproteomics : Methods in Molecular Biology. red. / Kelly M. Fulton; Susan M. Twine. Bind 1061 Humana Press, 2013. s. 167-79 (Methods in molecular biology (Clifton, N.J.)).

Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › peer review

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AU - Wandall, Hans H

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AB - Protein microarray is a highly sensitive tool for antibody detection in serum. Monitoring of patients' antibody titers to specific antigens is increasingly employed in the diagnosis of several conditions, ranging from infectious diseases, allergies, autoimmune diseases, and cancer. In this protocol we present a detailed method for enzymatic generation of disease-specific O-glycopeptides and how to monitor the antibody response to these in serum using microarray technology.

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KW - Antibodies

KW - Enzymes

KW - Glycopeptides

KW - Humans

KW - Protein Array Analysis

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PB - Humana Press

ER -

Chemo-enzymatic production of O-glycopeptides for the detection of serum glycopeptide antibodies

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