Cesarean section increases sensitivity to oxazolone-induced colitis in C57BL/6 mice

Line Fisker Zachariassen; Axel Kornerup Hansen; Lukasz Krych; Dennis Sandris Nielsen; Thomas Lindebo Holm; Peter Tougaard; Camilla Hartmann Friis Hansen

doi:10.1038/s41385-019-0207-8

Cesarean section increases sensitivity to oxazolone-induced colitis in C57BL/6 mice

Line Fisker Zachariassen^*, Axel Kornerup Hansen, Lukasz Krych, Dennis Sandris Nielsen, Thomas Lindebo Holm^*, Peter Tougaard, Camilla Hartmann Friis Hansen

^*Corresponding author for this work

Food Microbiology, Gut Health, and Fermentation

4 Citations (Scopus)

Abstract

Children born by cesarean section (CS) have an increased risk of developing inflammatory bowel disease (IBD), possibly due to skewed microbial colonization during birth and consequently impaired bacterial stimulation of the developing immune system. The aim of this study was to investigate the association between CS and experimental colitis in a murine model of IBD. It was hypothesized that CS aggravates colonic inflammation due to a change in gut microbiota (GM) composition. C57BL/6 mice, delivered by CS or vaginal delivery (VD), were intra-rectally challenged with oxazolone at 8 weeks of age and monitored for colitis symptoms. The results showed that CS delivered mice experienced an increased body weight loss and colon weight, together with higher colonic concentrations of TNF-α and MPO compared with VD mice. Increased infiltration of inflammatory cells was present in CS delivered mice, as well as a downregulation in expression of the gut integrity genes occludin and tight junction protein 1 indicative of an impaired barrier function. The GM from CS delivered mice without colitis partly contributed to the increase in colitis symptoms when inoculated into germ-free recipient mice. In conclusion, CS increased sensitivity to oxazolone induced colitis in mice.

Original language	English
Journal	Mucosal Immunology
Volume	12
Issue number	6
Pages (from-to)	1348-1357
Number of pages	10
ISSN	1933-0219
DOIs	https://doi.org/10.1038/s41385-019-0207-8
Publication status	Published - 2019

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title = "Cesarean section increases sensitivity to oxazolone-induced colitis in C57BL/6 mice",

abstract = "Children born by cesarean section (CS) have an increased risk of developing inflammatory bowel disease (IBD), possibly due to skewed microbial colonization during birth and consequently impaired bacterial stimulation of the developing immune system. The aim of this study was to investigate the association between CS and experimental colitis in a murine model of IBD. It was hypothesized that CS aggravates colonic inflammation due to a change in gut microbiota (GM) composition. C57BL/6 mice, delivered by CS or vaginal delivery (VD), were intra-rectally challenged with oxazolone at 8 weeks of age and monitored for colitis symptoms. The results showed that CS delivered mice experienced an increased body weight loss and colon weight, together with higher colonic concentrations of TNF-α and MPO compared with VD mice. Increased infiltration of inflammatory cells was present in CS delivered mice, as well as a downregulation in expression of the gut integrity genes occludin and tight junction protein 1 indicative of an impaired barrier function. The GM from CS delivered mice without colitis partly contributed to the increase in colitis symptoms when inoculated into germ-free recipient mice. In conclusion, CS increased sensitivity to oxazolone induced colitis in mice.",

author = "Zachariassen, {Line Fisker} and Hansen, {Axel Kornerup} and Lukasz Krych and Nielsen, {Dennis Sandris} and Holm, {Thomas Lindebo} and Peter Tougaard and Hansen, {Camilla Hartmann Friis}",

year = "2019",

doi = "10.1038/s41385-019-0207-8",

language = "English",

volume = "12",

pages = "1348--1357",

journal = "Mucosal Immunology",

issn = "1933-0219",

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number = "6",

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TY - JOUR

T1 - Cesarean section increases sensitivity to oxazolone-induced colitis in C57BL/6 mice

AU - Zachariassen, Line Fisker

AU - Hansen, Axel Kornerup

AU - Krych, Lukasz

AU - Nielsen, Dennis Sandris

AU - Holm, Thomas Lindebo

AU - Tougaard, Peter

AU - Hansen, Camilla Hartmann Friis

PY - 2019

Y1 - 2019

N2 - Children born by cesarean section (CS) have an increased risk of developing inflammatory bowel disease (IBD), possibly due to skewed microbial colonization during birth and consequently impaired bacterial stimulation of the developing immune system. The aim of this study was to investigate the association between CS and experimental colitis in a murine model of IBD. It was hypothesized that CS aggravates colonic inflammation due to a change in gut microbiota (GM) composition. C57BL/6 mice, delivered by CS or vaginal delivery (VD), were intra-rectally challenged with oxazolone at 8 weeks of age and monitored for colitis symptoms. The results showed that CS delivered mice experienced an increased body weight loss and colon weight, together with higher colonic concentrations of TNF-α and MPO compared with VD mice. Increased infiltration of inflammatory cells was present in CS delivered mice, as well as a downregulation in expression of the gut integrity genes occludin and tight junction protein 1 indicative of an impaired barrier function. The GM from CS delivered mice without colitis partly contributed to the increase in colitis symptoms when inoculated into germ-free recipient mice. In conclusion, CS increased sensitivity to oxazolone induced colitis in mice.

AB - Children born by cesarean section (CS) have an increased risk of developing inflammatory bowel disease (IBD), possibly due to skewed microbial colonization during birth and consequently impaired bacterial stimulation of the developing immune system. The aim of this study was to investigate the association between CS and experimental colitis in a murine model of IBD. It was hypothesized that CS aggravates colonic inflammation due to a change in gut microbiota (GM) composition. C57BL/6 mice, delivered by CS or vaginal delivery (VD), were intra-rectally challenged with oxazolone at 8 weeks of age and monitored for colitis symptoms. The results showed that CS delivered mice experienced an increased body weight loss and colon weight, together with higher colonic concentrations of TNF-α and MPO compared with VD mice. Increased infiltration of inflammatory cells was present in CS delivered mice, as well as a downregulation in expression of the gut integrity genes occludin and tight junction protein 1 indicative of an impaired barrier function. The GM from CS delivered mice without colitis partly contributed to the increase in colitis symptoms when inoculated into germ-free recipient mice. In conclusion, CS increased sensitivity to oxazolone induced colitis in mice.

U2 - 10.1038/s41385-019-0207-8

DO - 10.1038/s41385-019-0207-8

M3 - Journal article

C2 - 31554900

AN - SCOPUS:85074005748

SN - 1933-0219

VL - 12

SP - 1348

EP - 1357

JO - Mucosal Immunology

JF - Mucosal Immunology

IS - 6

ER -

Cesarean section increases sensitivity to oxazolone-induced colitis in C57BL/6 mice

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