Cell membrane conformation at vertical nanowire array interface revealed by fluorescence imaging

Trine Berthing; Sara Bonde; Katrine Rønne Rostgaard; Morten Hannibal Madsen; Claus Birger Sørensen; Jesper Nygård; Karen Laurence Martinez

doi:10.1088/0957-4484/23/41/415102

Cell membrane conformation at vertical nanowire array interface revealed by fluorescence imaging

Trine Berthing, Sara Bonde, Katrine Rønne Rostgaard, Morten Hannibal Madsen, Claus Birger Sørensen, Jesper Nygård, Karen Laurence Martinez

79 Citations (Scopus)

Abstract

The perspectives offered by vertical arrays of nanowires for biosensing applications in living cells depend on the access of individual nanowires to the cell interior. Recent results on electrical access and molecular delivery suggest that direct access is not always obtained. Here, we present a generic approach to directly visualize the membrane conformation of living cells interfaced with nanowire arrays, with single nanowire resolution. The method combines confocal z-stack imaging with an optimized cell membrane labelling strategy which was applied to HEK293 cells interfaced with 2-11μm long and 3-7μm spaced nanowires with various surface coatings (bare, aminosilane-coated or polyethyleneimine-coated indium arsenide). We demonstrate that, for all commonly used nanowire lengths, spacings and surface coatings, nanowires generally remain enclosed in a membrane compartment, and are thereby not in direct contact with the cell interior.

Original language	English
Journal	Nanotechnology
Volume	23
Issue number	41
Pages (from-to)	415102
ISSN	0957-4484
DOIs	https://doi.org/10.1088/0957-4484/23/41/415102
Publication status	Published - 19 Oct 2012

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title = "Cell membrane conformation at vertical nanowire array interface revealed by fluorescence imaging",

abstract = "The perspectives offered by vertical arrays of nanowires for biosensing applications in living cells depend on the access of individual nanowires to the cell interior. Recent results on electrical access and molecular delivery suggest that direct access is not always obtained. Here, we present a generic approach to directly visualize the membrane conformation of living cells interfaced with nanowire arrays, with single nanowire resolution. The method combines confocal z-stack imaging with an optimized cell membrane labelling strategy which was applied to HEK293 cells interfaced with 2-11μm long and 3-7μm spaced nanowires with various surface coatings (bare, aminosilane-coated or polyethyleneimine-coated indium arsenide). We demonstrate that, for all commonly used nanowire lengths, spacings and surface coatings, nanowires generally remain enclosed in a membrane compartment, and are thereby not in direct contact with the cell interior.",

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T1 - Cell membrane conformation at vertical nanowire array interface revealed by fluorescence imaging

AU - Berthing, Trine

AU - Bonde, Sara

AU - Rostgaard, Katrine Rønne

AU - Madsen, Morten Hannibal

AU - Sørensen, Claus Birger

AU - Nygård, Jesper

AU - Martinez, Karen Laurence

PY - 2012/10/19

Y1 - 2012/10/19

N2 - The perspectives offered by vertical arrays of nanowires for biosensing applications in living cells depend on the access of individual nanowires to the cell interior. Recent results on electrical access and molecular delivery suggest that direct access is not always obtained. Here, we present a generic approach to directly visualize the membrane conformation of living cells interfaced with nanowire arrays, with single nanowire resolution. The method combines confocal z-stack imaging with an optimized cell membrane labelling strategy which was applied to HEK293 cells interfaced with 2-11μm long and 3-7μm spaced nanowires with various surface coatings (bare, aminosilane-coated or polyethyleneimine-coated indium arsenide). We demonstrate that, for all commonly used nanowire lengths, spacings and surface coatings, nanowires generally remain enclosed in a membrane compartment, and are thereby not in direct contact with the cell interior.

AB - The perspectives offered by vertical arrays of nanowires for biosensing applications in living cells depend on the access of individual nanowires to the cell interior. Recent results on electrical access and molecular delivery suggest that direct access is not always obtained. Here, we present a generic approach to directly visualize the membrane conformation of living cells interfaced with nanowire arrays, with single nanowire resolution. The method combines confocal z-stack imaging with an optimized cell membrane labelling strategy which was applied to HEK293 cells interfaced with 2-11μm long and 3-7μm spaced nanowires with various surface coatings (bare, aminosilane-coated or polyethyleneimine-coated indium arsenide). We demonstrate that, for all commonly used nanowire lengths, spacings and surface coatings, nanowires generally remain enclosed in a membrane compartment, and are thereby not in direct contact with the cell interior.

U2 - 10.1088/0957-4484/23/41/415102

DO - 10.1088/0957-4484/23/41/415102

M3 - Journal article

C2 - 23010859

SN - 0957-4484

VL - 23

SP - 415102

JO - Nanotechnology

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Cell membrane conformation at vertical nanowire array interface revealed by fluorescence imaging

Abstract

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