Cell biology of homologous recombination in yeast

Nadine Valerie Eckert-Boulet, Rodney Rothstein, Michael Lisby

22 Citations (Scopus)

Abstract

Homologous recombination is an important pathway for error-free repair of DNA lesions, such as single- and double-strand breaks, and for rescue of collapsed replication forks. Here, we describe protocols for live cell imaging of single-lesion recombination events in the yeast Saccharomyces cerevisiae using fluorescence microscopy.
Original languageEnglish
Title of host publicationDNA recombination : methods and protocols
EditorsHideo Tsubouchi
Number of pages14
PublisherSpringer
Publication date2011
Pages523-536
DOIs
Publication statusPublished - 2011
SeriesMethods in Molecular Biology
Volume745
ISSN1064-3745

Keywords

  • DNA Breaks, Double-Stranded
  • DNA Damage
  • Microscopy, Fluorescence
  • Recombination, Genetic
  • Saccharomyces cerevisiae

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