Abstract
Caveolae are abundant in skeletal muscle and their coat contains a specific isoform of caveolin, caveolin-3. It has been suggested that during muscle development, caveolin-3 is associated with the T-tubules, but that in adult muscle it is found on the plasma membrane only. We have studied the distribution of caveolin-3 in single skeletal muscle fibers from adult rat soleus by confocal immunofluorescence and by immunogold electron microscopy. We found that caveolin-3 occurs at the highest density on the plasma membrane but is also present in the core of the fibers, at the I-band/A-band interface where it is associated with the T-tubules. In neither domain of the muscle surface does caveolin-3 colocalize with the glucose transporter GLUT4 and there is no evidence for internalization of the caveolae in muscle.
| Original language | English |
|---|---|
| Journal | Experimental Cell Research |
| Volume | 246 |
| Issue number | 2 |
| Pages (from-to) | 510-5 |
| Number of pages | 6 |
| ISSN | 0014-4827 |
| DOIs | |
| Publication status | Published - 1 Feb 1999 |
Keywords
- Animals
- Caveolin 3
- Caveolins
- Membrane Proteins
- Mice
- Muscle Fibers, Skeletal
- Muscle, Skeletal
- Rabbits
- Rats
- Rats, Wistar