Abstract
Caveolae are abundant in skeletal muscle and their coat contains a specific isoform of caveolin, caveolin-3. It has been suggested that during muscle development, caveolin-3 is associated with the T-tubules, but that in adult muscle it is found on the plasma membrane only. We have studied the distribution of caveolin-3 in single skeletal muscle fibers from adult rat soleus by confocal immunofluorescence and by immunogold electron microscopy. We found that caveolin-3 occurs at the highest density on the plasma membrane but is also present in the core of the fibers, at the I-band/A-band interface where it is associated with the T-tubules. In neither domain of the muscle surface does caveolin-3 colocalize with the glucose transporter GLUT4 and there is no evidence for internalization of the caveolae in muscle.
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Experimental Cell Research |
| Vol/bind | 246 |
| Udgave nummer | 2 |
| Sider (fra-til) | 510-5 |
| Antal sider | 6 |
| ISSN | 0014-4827 |
| DOI | |
| Status | Udgivet - 1 feb. 1999 |