Abstract
Inhibition of the constitutively active casein kinase 2 (CK2) with 2-dimethyl-amino-4,5,6,7-tetrabromo-1H-benzimidasole stimulates the Na(+)-dependent taurine influx via the taurine transporter TauT in NIH3T3 cells. CK2 inhibition reduces the TauT mRNA level and increases the localization of TauT to ER but has no detectable effect on TauT protein expression. On the other hand, CK2 inhibition increases the affinity of TauT towards Na(+ )and reduces the Na(+)/taurine stoichiometry for active taurine uptake. It is suggested that CK2 controls the cellular taurine uptake in unperturbated NIH3T3 cells, i.e., inhibition of CK2 increases the affinity of TauT towards Na(+) and hence Na(+)-dependent taurine uptake.
| Original language | English |
|---|---|
| Journal | Pflügers Archiv - European Journal of Physiology |
| Volume | 457 |
| Issue number | 2 |
| Pages (from-to) | 327-37 |
| Number of pages | 11 |
| ISSN | 0031-6768 |
| DOIs | |
| Publication status | Published - 1 Nov 2008 |
Keywords
- Animals
- Benzimidazoles
- Biological Transport, Active
- Casein Kinase II
- Endoplasmic Reticulum
- Fibroblasts
- Kinetics
- Membrane Glycoproteins
- Membrane Transport Proteins
- Mice
- NIH 3T3 Cells
- Protein Kinase Inhibitors
- Protein Transport
- RNA, Messenger
- Sodium
- Taurine
- Water-Electrolyte Balance