Casein kinase 2 regulates the active uptake of the organic osmolyte taurine in NIH3T3 mouse fibroblasts

TY - JOUR

T1 - Casein kinase 2 regulates the active uptake of the organic osmolyte taurine in NIH3T3 mouse fibroblasts

AU - Jacobsen, Jack H

AU - Clement, Christian A

AU - Friis, Martin B

AU - Lambert, Ian H

PY - 2008/11/1

Y1 - 2008/11/1

N2 - Inhibition of the constitutively active casein kinase 2 (CK2) with 2-dimethyl-amino-4,5,6,7-tetrabromo-1H-benzimidasole stimulates the Na(+)-dependent taurine influx via the taurine transporter TauT in NIH3T3 cells. CK2 inhibition reduces the TauT mRNA level and increases the localization of TauT to ER but has no detectable effect on TauT protein expression. On the other hand, CK2 inhibition increases the affinity of TauT towards Na(+ )and reduces the Na(+)/taurine stoichiometry for active taurine uptake. It is suggested that CK2 controls the cellular taurine uptake in unperturbated NIH3T3 cells, i.e., inhibition of CK2 increases the affinity of TauT towards Na(+) and hence Na(+)-dependent taurine uptake.

AB - Inhibition of the constitutively active casein kinase 2 (CK2) with 2-dimethyl-amino-4,5,6,7-tetrabromo-1H-benzimidasole stimulates the Na(+)-dependent taurine influx via the taurine transporter TauT in NIH3T3 cells. CK2 inhibition reduces the TauT mRNA level and increases the localization of TauT to ER but has no detectable effect on TauT protein expression. On the other hand, CK2 inhibition increases the affinity of TauT towards Na(+ )and reduces the Na(+)/taurine stoichiometry for active taurine uptake. It is suggested that CK2 controls the cellular taurine uptake in unperturbated NIH3T3 cells, i.e., inhibition of CK2 increases the affinity of TauT towards Na(+) and hence Na(+)-dependent taurine uptake.

KW - Animals

KW - Benzimidazoles

KW - Biological Transport, Active

KW - Casein Kinase II

KW - Endoplasmic Reticulum

KW - Fibroblasts

KW - Kinetics

KW - Membrane Glycoproteins

KW - Membrane Transport Proteins

KW - Mice

KW - NIH 3T3 Cells

KW - Protein Kinase Inhibitors

KW - Protein Transport

KW - RNA, Messenger

KW - Sodium

KW - Taurine

KW - Water-Electrolyte Balance

U2 - 10.1007/s00424-008-0517-2

DO - 10.1007/s00424-008-0517-2

M3 - Journal article

C2 - 18542993

SN - 0031-6768

VL - 457

SP - 327

EP - 337

JO - Pflügers Archiv - European Journal of Physiology

JF - Pflügers Archiv - European Journal of Physiology

IS - 2

ER -