Carbohydrate microarrays in plant science

Jonatan Ulrik Fangel; Henriette Lodberg Pedersen; Silvia Vidal Melgosa; Louise Isager Ahl; Armando Asunción Salmeán; Jack Egelund Madsen; Maja Gro Rydahl; Mads Hartvig Clausen; William George Tycho Willats

doi:10.1007/978-1-61779-995-2_19

Carbohydrate microarrays in plant science

Jonatan Ulrik Fangel, Henriette Lodberg Pedersen, Silvia Vidal Melgosa, Louise Isager Ahl, Armando Asunción Salmeán, Jack Egelund Madsen, Maja Gro Rydahl, Mads Hartvig Clausen, William George Tycho Willats

13 Citations (Scopus)

Abstract

Almost all plant cells are surrounded by glycan-rich cell walls, which form much of the plant body and collectively are the largest source of biomass on earth. Plants use polysaccharides for support, defense, signaling, cell adhesion, and as energy storage, and many plant glycans are also important industrially and nutritionally. Understanding the biological roles of plant glycans and the effective exploitation of their useful properties requires a detailed understanding of their structures, occurrence, and molecular interactions. Microarray technology has revolutionized the massively high-throughput analysis of nucleotides, proteins, and increasingly carbohydrates. Using microarrays, the abundance of and interactions between hundreds and thousands of molecules can be assessed simultaneously using very small amounts of analytes. Here we show that carbohydrate microarrays are multifunctional tools for plant research and can be used to map glycan populations across large numbers of samples to screen antibodies, carbohydrate binding proteins, and carbohydrate binding modules and to investigate enzyme activities.

Original language	English
Title of host publication	High-throughput phenotyping in plants : methods and protocols
Editors	Jennifer Normanly
Number of pages	12
Publisher	Springer
Publication date	2012
Pages	351-362
ISBN (Print)	978-1-61779-994-5
ISBN (Electronic)	978-1-61779-995-2
DOIs	https://doi.org/10.1007/978-1-61779-995-2_19
Publication status	Published - 2012

Series	Methods in Molecular Biology
Volume	918
ISSN	1064-3745

Keywords

Carbohydrates
Microarray Analysis
Oligosaccharides
Plants

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10.1007/978-1-61779-995-2_19

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Carbohydrate microarrays in plant science. / Fangel, Jonatan Ulrik; Pedersen, Henriette Lodberg; Vidal Melgosa, Silvia et al.
High-throughput phenotyping in plants: methods and protocols. ed. / Jennifer Normanly. Springer, 2012. p. 351-362 (Methods in Molecular Biology, Vol. 918).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

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AB - Almost all plant cells are surrounded by glycan-rich cell walls, which form much of the plant body and collectively are the largest source of biomass on earth. Plants use polysaccharides for support, defense, signaling, cell adhesion, and as energy storage, and many plant glycans are also important industrially and nutritionally. Understanding the biological roles of plant glycans and the effective exploitation of their useful properties requires a detailed understanding of their structures, occurrence, and molecular interactions. Microarray technology has revolutionized the massively high-throughput analysis of nucleotides, proteins, and increasingly carbohydrates. Using microarrays, the abundance of and interactions between hundreds and thousands of molecules can be assessed simultaneously using very small amounts of analytes. Here we show that carbohydrate microarrays are multifunctional tools for plant research and can be used to map glycan populations across large numbers of samples to screen antibodies, carbohydrate binding proteins, and carbohydrate binding modules and to investigate enzyme activities.

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Carbohydrate microarrays in plant science

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Keywords

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