TY - CHAP
T1 - Carbohydrate microarrays in plant science
AU - Fangel, Jonatan Ulrik
AU - Pedersen, Henriette Lodberg
AU - Vidal Melgosa, Silvia
AU - Ahl, Louise Isager
AU - Asunción Salmeán, Armando
AU - Madsen, Jack Egelund
AU - Rydahl, Maja Gro
AU - Clausen, Mads Hartvig
AU - Willats, William George Tycho
PY - 2012
Y1 - 2012
N2 - Almost all plant cells are surrounded by glycan-rich cell walls, which form much of the plant body and collectively are the largest source of biomass on earth. Plants use polysaccharides for support, defense, signaling, cell adhesion, and as energy storage, and many plant glycans are also important industrially and nutritionally. Understanding the biological roles of plant glycans and the effective exploitation of their useful properties requires a detailed understanding of their structures, occurrence, and molecular interactions. Microarray technology has revolutionized the massively high-throughput analysis of nucleotides, proteins, and increasingly carbohydrates. Using microarrays, the abundance of and interactions between hundreds and thousands of molecules can be assessed simultaneously using very small amounts of analytes. Here we show that carbohydrate microarrays are multifunctional tools for plant research and can be used to map glycan populations across large numbers of samples to screen antibodies, carbohydrate binding proteins, and carbohydrate binding modules and to investigate enzyme activities.
AB - Almost all plant cells are surrounded by glycan-rich cell walls, which form much of the plant body and collectively are the largest source of biomass on earth. Plants use polysaccharides for support, defense, signaling, cell adhesion, and as energy storage, and many plant glycans are also important industrially and nutritionally. Understanding the biological roles of plant glycans and the effective exploitation of their useful properties requires a detailed understanding of their structures, occurrence, and molecular interactions. Microarray technology has revolutionized the massively high-throughput analysis of nucleotides, proteins, and increasingly carbohydrates. Using microarrays, the abundance of and interactions between hundreds and thousands of molecules can be assessed simultaneously using very small amounts of analytes. Here we show that carbohydrate microarrays are multifunctional tools for plant research and can be used to map glycan populations across large numbers of samples to screen antibodies, carbohydrate binding proteins, and carbohydrate binding modules and to investigate enzyme activities.
KW - Carbohydrates
KW - Microarray Analysis
KW - Oligosaccharides
KW - Plants
U2 - 10.1007/978-1-61779-995-2_19
DO - 10.1007/978-1-61779-995-2_19
M3 - Book chapter
C2 - 22893299
SN - 978-1-61779-994-5
T3 - Methods in Molecular Biology
SP - 351
EP - 362
BT - High-throughput phenotyping in plants
A2 - Normanly, Jennifer
PB - Springer
ER -