Carbohydrate microarrays in plant science

Jonatan Ulrik Fangel; Henriette Lodberg Pedersen; Silvia Vidal Melgosa; Louise Isager Ahl; Armando Asunción Salmeán; Jack Egelund Madsen; Maja Gro Rydahl; Mads Hartvig Clausen; William George Tycho Willats

doi:10.1007/978-1-61779-995-2_19

Carbohydrate microarrays in plant science

Jonatan Ulrik Fangel, Henriette Lodberg Pedersen, Silvia Vidal Melgosa, Louise Isager Ahl, Armando Asunción Salmeán, Jack Egelund Madsen, Maja Gro Rydahl, Mads Hartvig Clausen, William George Tycho Willats

13 Citationer (Scopus)

Abstract

Almost all plant cells are surrounded by glycan-rich cell walls, which form much of the plant body and collectively are the largest source of biomass on earth. Plants use polysaccharides for support, defense, signaling, cell adhesion, and as energy storage, and many plant glycans are also important industrially and nutritionally. Understanding the biological roles of plant glycans and the effective exploitation of their useful properties requires a detailed understanding of their structures, occurrence, and molecular interactions. Microarray technology has revolutionized the massively high-throughput analysis of nucleotides, proteins, and increasingly carbohydrates. Using microarrays, the abundance of and interactions between hundreds and thousands of molecules can be assessed simultaneously using very small amounts of analytes. Here we show that carbohydrate microarrays are multifunctional tools for plant research and can be used to map glycan populations across large numbers of samples to screen antibodies, carbohydrate binding proteins, and carbohydrate binding modules and to investigate enzyme activities.

Originalsprog	Engelsk
Titel	High-throughput phenotyping in plants : methods and protocols
Redaktører	Jennifer Normanly
Antal sider	12
Forlag	Springer
Publikationsdato	2012
Sider	351-362
ISBN (Trykt)	978-1-61779-994-5
ISBN (Elektronisk)	978-1-61779-995-2
DOI	https://doi.org/10.1007/978-1-61779-995-2_19
Status	Udgivet - 2012

Navn	Methods in Molecular Biology
Vol/bind	918
ISSN	1064-3745

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10.1007/978-1-61779-995-2_19

Citationsformater

Fangel, J. U., Pedersen, H. L., Vidal Melgosa, S., Ahl, L. I., Asunción Salmeán, A., Madsen, J. E., Rydahl, M. G., Clausen, M. H., & Willats, W. G. T. (2012). Carbohydrate microarrays in plant science. I J. Normanly (red.), High-throughput phenotyping in plants: methods and protocols (s. 351-362). Springer. Methods in Molecular Biology Bind 918 https://doi.org/10.1007/978-1-61779-995-2_19

Carbohydrate microarrays in plant science. / Fangel, Jonatan Ulrik; Pedersen, Henriette Lodberg; Vidal Melgosa, Silvia et al.

High-throughput phenotyping in plants: methods and protocols. red. / Jennifer Normanly. Springer, 2012. s. 351-362 (Methods in Molecular Biology, Bind 918).

Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › peer review

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abstract = "Almost all plant cells are surrounded by glycan-rich cell walls, which form much of the plant body and collectively are the largest source of biomass on earth. Plants use polysaccharides for support, defense, signaling, cell adhesion, and as energy storage, and many plant glycans are also important industrially and nutritionally. Understanding the biological roles of plant glycans and the effective exploitation of their useful properties requires a detailed understanding of their structures, occurrence, and molecular interactions. Microarray technology has revolutionized the massively high-throughput analysis of nucleotides, proteins, and increasingly carbohydrates. Using microarrays, the abundance of and interactions between hundreds and thousands of molecules can be assessed simultaneously using very small amounts of analytes. Here we show that carbohydrate microarrays are multifunctional tools for plant research and can be used to map glycan populations across large numbers of samples to screen antibodies, carbohydrate binding proteins, and carbohydrate binding modules and to investigate enzyme activities.",

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AU - Madsen, Jack Egelund

AU - Rydahl, Maja Gro

AU - Clausen, Mads Hartvig

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AB - Almost all plant cells are surrounded by glycan-rich cell walls, which form much of the plant body and collectively are the largest source of biomass on earth. Plants use polysaccharides for support, defense, signaling, cell adhesion, and as energy storage, and many plant glycans are also important industrially and nutritionally. Understanding the biological roles of plant glycans and the effective exploitation of their useful properties requires a detailed understanding of their structures, occurrence, and molecular interactions. Microarray technology has revolutionized the massively high-throughput analysis of nucleotides, proteins, and increasingly carbohydrates. Using microarrays, the abundance of and interactions between hundreds and thousands of molecules can be assessed simultaneously using very small amounts of analytes. Here we show that carbohydrate microarrays are multifunctional tools for plant research and can be used to map glycan populations across large numbers of samples to screen antibodies, carbohydrate binding proteins, and carbohydrate binding modules and to investigate enzyme activities.

KW - Carbohydrates

KW - Microarray Analysis

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Carbohydrate microarrays in plant science

Abstract

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