Brazilian insulin plant as a bifunctional food: Dual high-resolution PTP1B and α-glucosidase inhibition profiling combined with HPLC-HRMS-SPE-NMR for identification of antidiabetic compounds in Myrcia rubella Cambess

Rita de Cassia Lemos Lima; Lucilia Kato; Kenneth Thermann Kongstad; Dan Stærk

doi:10.1016/j.jff.2018.04.019

Brazilian insulin plant as a bifunctional food: Dual high-resolution PTP1B and α-glucosidase inhibition profiling combined with HPLC-HRMS-SPE-NMR for identification of antidiabetic compounds in Myrcia rubella Cambess

Rita de Cassia Lemos Lima, Lucilia Kato, Kenneth Thermann Kongstad, Dan Stærk

10 Citations (Scopus)

Abstract

Six species of Myrcia, commonly known as 'insulin plants' in Brazil, were assessed for their potential as functional foods. Thus, dual high-resolution α-glucosidase/PTP1B inhibition profiling and HPLC-HRMS-SPE-NMR analysis were used to identify the antidiabetic constituents of the crude ethyl acetate extract of M. rubella Cambess. A total of 31 compounds were identified, including seven α-glucosidase inhibitors with IC₅₀ values between 0.6 and 22.4 μM: 4,5-dicaffeoylquinic acid, isoquercitrin, quercetin-3-O-β-D-glucuronide, kaempferol-3-O-(6″-galloyl)-β-D-glucopyranoside, quercetin-3-O-(6″-malonyl)-glucopyranoside, quercetin-3-O-(6″-(E)-feruloyl)-β-D-glucopyranoside, and quercetin-3-O-(2″-(E)-sinapoyl)-glucopyranoside as well as three strong PTP1B inhibitors with IC₅₀ values between 0.4 and 3.1 μM: kaempferol-3-O-α-L-rhamnoside, astragalin, and arjunolic acid. These results show that M. rubella is a potential bifunctional food for management of type 2 diabetes.

Original language	English
Journal	Journal of Functional Foods
Volume	45
Pages (from-to)	444-451
ISSN	1756-4646
DOIs	https://doi.org/10.1016/j.jff.2018.04.019
Publication status	Published - Jun 2018

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Brazilian insulin plant as a bifunctional food: Dual high-resolution PTP1B and α-glucosidase inhibition profiling combined with HPLC-HRMS-SPE-NMR for identification of antidiabetic compounds in Myrcia rubella Cambess. / Lima, Rita de Cassia Lemos; Kato, Lucilia; Kongstad, Kenneth Thermann et al.

In: Journal of Functional Foods, Vol. 45, 06.2018, p. 444-451.

Research output: Contribution to journal › Journal article › Research › peer-review

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title = "Brazilian insulin plant as a bifunctional food: Dual high-resolution PTP1B and α-glucosidase inhibition profiling combined with HPLC-HRMS-SPE-NMR for identification of antidiabetic compounds in Myrcia rubella Cambess",

abstract = "Six species of Myrcia, commonly known as 'insulin plants' in Brazil, were assessed for their potential as functional foods. Thus, dual high-resolution α-glucosidase/PTP1B inhibition profiling and HPLC-HRMS-SPE-NMR analysis were used to identify the antidiabetic constituents of the crude ethyl acetate extract of M. rubella Cambess. A total of 31 compounds were identified, including seven α-glucosidase inhibitors with IC50 values between 0.6 and 22.4 μM: 4,5-dicaffeoylquinic acid, isoquercitrin, quercetin-3-O-β-D-glucuronide, kaempferol-3-O-(6″-galloyl)-β-D-glucopyranoside, quercetin-3-O-(6″-malonyl)-glucopyranoside, quercetin-3-O-(6″-(E)-feruloyl)-β-D-glucopyranoside, and quercetin-3-O-(2″-(E)-sinapoyl)-glucopyranoside as well as three strong PTP1B inhibitors with IC50 values between 0.4 and 3.1 μM: kaempferol-3-O-α-L-rhamnoside, astragalin, and arjunolic acid. These results show that M. rubella is a potential bifunctional food for management of type 2 diabetes.",

author = "Lima, {Rita de Cassia Lemos} and Lucilia Kato and Kongstad, {Kenneth Thermann} and Dan St{\ae}rk",

year = "2018",

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AU - Lima, Rita de Cassia Lemos

AU - Kato, Lucilia

AU - Kongstad, Kenneth Thermann

AU - Stærk, Dan

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N2 - Six species of Myrcia, commonly known as 'insulin plants' in Brazil, were assessed for their potential as functional foods. Thus, dual high-resolution α-glucosidase/PTP1B inhibition profiling and HPLC-HRMS-SPE-NMR analysis were used to identify the antidiabetic constituents of the crude ethyl acetate extract of M. rubella Cambess. A total of 31 compounds were identified, including seven α-glucosidase inhibitors with IC50 values between 0.6 and 22.4 μM: 4,5-dicaffeoylquinic acid, isoquercitrin, quercetin-3-O-β-D-glucuronide, kaempferol-3-O-(6″-galloyl)-β-D-glucopyranoside, quercetin-3-O-(6″-malonyl)-glucopyranoside, quercetin-3-O-(6″-(E)-feruloyl)-β-D-glucopyranoside, and quercetin-3-O-(2″-(E)-sinapoyl)-glucopyranoside as well as three strong PTP1B inhibitors with IC50 values between 0.4 and 3.1 μM: kaempferol-3-O-α-L-rhamnoside, astragalin, and arjunolic acid. These results show that M. rubella is a potential bifunctional food for management of type 2 diabetes.

AB - Six species of Myrcia, commonly known as 'insulin plants' in Brazil, were assessed for their potential as functional foods. Thus, dual high-resolution α-glucosidase/PTP1B inhibition profiling and HPLC-HRMS-SPE-NMR analysis were used to identify the antidiabetic constituents of the crude ethyl acetate extract of M. rubella Cambess. A total of 31 compounds were identified, including seven α-glucosidase inhibitors with IC50 values between 0.6 and 22.4 μM: 4,5-dicaffeoylquinic acid, isoquercitrin, quercetin-3-O-β-D-glucuronide, kaempferol-3-O-(6″-galloyl)-β-D-glucopyranoside, quercetin-3-O-(6″-malonyl)-glucopyranoside, quercetin-3-O-(6″-(E)-feruloyl)-β-D-glucopyranoside, and quercetin-3-O-(2″-(E)-sinapoyl)-glucopyranoside as well as three strong PTP1B inhibitors with IC50 values between 0.4 and 3.1 μM: kaempferol-3-O-α-L-rhamnoside, astragalin, and arjunolic acid. These results show that M. rubella is a potential bifunctional food for management of type 2 diabetes.

U2 - 10.1016/j.jff.2018.04.019

DO - 10.1016/j.jff.2018.04.019

M3 - Journal article

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