Brazilian insulin plant as a bifunctional food: Dual high-resolution PTP1B and α-glucosidase inhibition profiling combined with HPLC-HRMS-SPE-NMR for identification of antidiabetic compounds in Myrcia rubella Cambess

Rita de Cassia Lemos Lima; Lucilia Kato; Kenneth Thermann Kongstad; Dan Stærk

doi:10.1016/j.jff.2018.04.019

Brazilian insulin plant as a bifunctional food: Dual high-resolution PTP1B and α-glucosidase inhibition profiling combined with HPLC-HRMS-SPE-NMR for identification of antidiabetic compounds in Myrcia rubella Cambess

Rita de Cassia Lemos Lima, Lucilia Kato, Kenneth Thermann Kongstad, Dan Stærk

10 Citationer (Scopus)

Abstract

Six species of Myrcia, commonly known as 'insulin plants' in Brazil, were assessed for their potential as functional foods. Thus, dual high-resolution α-glucosidase/PTP1B inhibition profiling and HPLC-HRMS-SPE-NMR analysis were used to identify the antidiabetic constituents of the crude ethyl acetate extract of M. rubella Cambess. A total of 31 compounds were identified, including seven α-glucosidase inhibitors with IC₅₀ values between 0.6 and 22.4 μM: 4,5-dicaffeoylquinic acid, isoquercitrin, quercetin-3-O-β-D-glucuronide, kaempferol-3-O-(6″-galloyl)-β-D-glucopyranoside, quercetin-3-O-(6″-malonyl)-glucopyranoside, quercetin-3-O-(6″-(E)-feruloyl)-β-D-glucopyranoside, and quercetin-3-O-(2″-(E)-sinapoyl)-glucopyranoside as well as three strong PTP1B inhibitors with IC₅₀ values between 0.4 and 3.1 μM: kaempferol-3-O-α-L-rhamnoside, astragalin, and arjunolic acid. These results show that M. rubella is a potential bifunctional food for management of type 2 diabetes.

Originalsprog	Engelsk
Tidsskrift	Journal of Functional Foods
Vol/bind	45
Sider (fra-til)	444-451
ISSN	1756-4646
DOI	https://doi.org/10.1016/j.jff.2018.04.019
Status	Udgivet - jun. 2018

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Brazilian insulin plant as a bifunctional food: Dual high-resolution PTP1B and α-glucosidase inhibition profiling combined with HPLC-HRMS-SPE-NMR for identification of antidiabetic compounds in Myrcia rubella Cambess. / Lima, Rita de Cassia Lemos; Kato, Lucilia; Kongstad, Kenneth Thermann et al.
I: Journal of Functional Foods, Bind 45, 06.2018, s. 444-451.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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title = "Brazilian insulin plant as a bifunctional food: Dual high-resolution PTP1B and α-glucosidase inhibition profiling combined with HPLC-HRMS-SPE-NMR for identification of antidiabetic compounds in Myrcia rubella Cambess",

abstract = "Six species of Myrcia, commonly known as 'insulin plants' in Brazil, were assessed for their potential as functional foods. Thus, dual high-resolution α-glucosidase/PTP1B inhibition profiling and HPLC-HRMS-SPE-NMR analysis were used to identify the antidiabetic constituents of the crude ethyl acetate extract of M. rubella Cambess. A total of 31 compounds were identified, including seven α-glucosidase inhibitors with IC50 values between 0.6 and 22.4 μM: 4,5-dicaffeoylquinic acid, isoquercitrin, quercetin-3-O-β-D-glucuronide, kaempferol-3-O-(6″-galloyl)-β-D-glucopyranoside, quercetin-3-O-(6″-malonyl)-glucopyranoside, quercetin-3-O-(6″-(E)-feruloyl)-β-D-glucopyranoside, and quercetin-3-O-(2″-(E)-sinapoyl)-glucopyranoside as well as three strong PTP1B inhibitors with IC50 values between 0.4 and 3.1 μM: kaempferol-3-O-α-L-rhamnoside, astragalin, and arjunolic acid. These results show that M. rubella is a potential bifunctional food for management of type 2 diabetes.",

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AU - Kato, Lucilia

AU - Kongstad, Kenneth Thermann

AU - Stærk, Dan

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N2 - Six species of Myrcia, commonly known as 'insulin plants' in Brazil, were assessed for their potential as functional foods. Thus, dual high-resolution α-glucosidase/PTP1B inhibition profiling and HPLC-HRMS-SPE-NMR analysis were used to identify the antidiabetic constituents of the crude ethyl acetate extract of M. rubella Cambess. A total of 31 compounds were identified, including seven α-glucosidase inhibitors with IC50 values between 0.6 and 22.4 μM: 4,5-dicaffeoylquinic acid, isoquercitrin, quercetin-3-O-β-D-glucuronide, kaempferol-3-O-(6″-galloyl)-β-D-glucopyranoside, quercetin-3-O-(6″-malonyl)-glucopyranoside, quercetin-3-O-(6″-(E)-feruloyl)-β-D-glucopyranoside, and quercetin-3-O-(2″-(E)-sinapoyl)-glucopyranoside as well as three strong PTP1B inhibitors with IC50 values between 0.4 and 3.1 μM: kaempferol-3-O-α-L-rhamnoside, astragalin, and arjunolic acid. These results show that M. rubella is a potential bifunctional food for management of type 2 diabetes.

AB - Six species of Myrcia, commonly known as 'insulin plants' in Brazil, were assessed for their potential as functional foods. Thus, dual high-resolution α-glucosidase/PTP1B inhibition profiling and HPLC-HRMS-SPE-NMR analysis were used to identify the antidiabetic constituents of the crude ethyl acetate extract of M. rubella Cambess. A total of 31 compounds were identified, including seven α-glucosidase inhibitors with IC50 values between 0.6 and 22.4 μM: 4,5-dicaffeoylquinic acid, isoquercitrin, quercetin-3-O-β-D-glucuronide, kaempferol-3-O-(6″-galloyl)-β-D-glucopyranoside, quercetin-3-O-(6″-malonyl)-glucopyranoside, quercetin-3-O-(6″-(E)-feruloyl)-β-D-glucopyranoside, and quercetin-3-O-(2″-(E)-sinapoyl)-glucopyranoside as well as three strong PTP1B inhibitors with IC50 values between 0.4 and 3.1 μM: kaempferol-3-O-α-L-rhamnoside, astragalin, and arjunolic acid. These results show that M. rubella is a potential bifunctional food for management of type 2 diabetes.

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