Abstract
We describe a technique which permits an easy screening for amber mutants defective in essential genes of Escherichia coli. Using this approach, we have isolated three amber mutants defective in the rho gene. An extension of the technique allows the detection of ochre mutants and transposon insertions in essential genes.
| Original language | English |
|---|---|
| Journal | Journal of Bacteriology |
| Volume | 146 |
| Issue number | 1 |
| Pages (from-to) | 422-5 |
| Number of pages | 3 |
| ISSN | 0021-9193 |
| Publication status | Published - 1981 |