Blue ghosts: a new method for isolating amber mutants defective in essential genes of Escherichia coli

S Brown; E R Brickman; J Beckwith

Blue ghosts: a new method for isolating amber mutants defective in essential genes of Escherichia coli

S Brown, E R Brickman, J Beckwith

Bioinformatik og RNA Biologi

15 Citationer (Scopus)

Abstract

Udgivelsesdato: 1981-Apr

Originalsprog	Engelsk
Tidsskrift	Journal of Bacteriology
Vol/bind	146
Udgave nummer	1
Sider (fra-til)	422-5
Antal sider	3
ISSN	0021-9193
Status	Udgivet - 1981

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http://jb.asm.org/cgi/reprint/146/1/422?view=long&pmid=7012125

Citationsformater

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title = "Blue ghosts: a new method for isolating amber mutants defective in essential genes of Escherichia coli",

abstract = "We describe a technique which permits an easy screening for amber mutants defective in essential genes of Escherichia coli. Using this approach, we have isolated three amber mutants defective in the rho gene. An extension of the technique allows the detection of ochre mutants and transposon insertions in essential genes.",

author = "S Brown and Brickman, {E R} and J Beckwith",

note = "Keywords: Escherichia coli; Genes; Genetic Techniques; Mutation",

year = "1981",

language = "English",

volume = "146",

pages = "422--5",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

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TY - JOUR

T1 - Blue ghosts: a new method for isolating amber mutants defective in essential genes of Escherichia coli

AU - Brown, S

AU - Brickman, E R

AU - Beckwith, J

N1 - Keywords: Escherichia coli; Genes; Genetic Techniques; Mutation

PY - 1981

Y1 - 1981

N2 - We describe a technique which permits an easy screening for amber mutants defective in essential genes of Escherichia coli. Using this approach, we have isolated three amber mutants defective in the rho gene. An extension of the technique allows the detection of ochre mutants and transposon insertions in essential genes.

AB - We describe a technique which permits an easy screening for amber mutants defective in essential genes of Escherichia coli. Using this approach, we have isolated three amber mutants defective in the rho gene. An extension of the technique allows the detection of ochre mutants and transposon insertions in essential genes.

M3 - Journal article

C2 - 7012125

SN - 0021-9193

VL - 146

SP - 422

EP - 425

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 1

ER -

Blue ghosts: a new method for isolating amber mutants defective in essential genes of Escherichia coli

Abstract

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Citationsformater