Biomimetic triblock copolymer membrane arrays: A stable template for functional membrane proteins

Alfredo González-Pérez; Karin B. Stibius; Thomas Vissing; Claus H. Nielsen; Ole G. Mouritsen

doi:10.1021/la902417m

Biomimetic triblock copolymer membrane arrays: A stable template for functional membrane proteins

Alfredo González-Pérez^*, Karin B. Stibius, Thomas Vissing, Claus H. Nielsen, Ole G. Mouritsen

^*Corresponding author for this work

55 Citations (Scopus)

Abstract

It is demonstrated that biomimetic stable triblock copolymer membrane arrays can be prepared using a scaffold containing 64 apertures of 300 μm diameter each. The membranes were made from a stock solution of block copolymers with decane as a solvent using a new deposition method. By using decane, we avoid low molecular weight solvents such as chloroform and toluene, which are strong protein denaturants. The membranes show a low ionic conductance and a long lifetime at room temperature. Contrast phase microscopy shows the presence of a polymer region delimited by a Plateau-Gibbs border similar to what is observed in black lipid membranes. The ion-channel gramicidin A was successfully incorporated into the membrane in a functional form.

Original language	English
Journal	Langmuir
Volume	25
Issue number	18
Pages (from-to)	10447-10450
Number of pages	4
ISSN	0743-7463
DOIs	https://doi.org/10.1021/la902417m
Publication status	Published - 15 Sept 2009

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abstract = "It is demonstrated that biomimetic stable triblock copolymer membrane arrays can be prepared using a scaffold containing 64 apertures of 300 μm diameter each. The membranes were made from a stock solution of block copolymers with decane as a solvent using a new deposition method. By using decane, we avoid low molecular weight solvents such as chloroform and toluene, which are strong protein denaturants. The membranes show a low ionic conductance and a long lifetime at room temperature. Contrast phase microscopy shows the presence of a polymer region delimited by a Plateau-Gibbs border similar to what is observed in black lipid membranes. The ion-channel gramicidin A was successfully incorporated into the membrane in a functional form.",

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AU - González-Pérez, Alfredo

AU - Stibius, Karin B.

AU - Vissing, Thomas

AU - Nielsen, Claus H.

AU - Mouritsen, Ole G.

PY - 2009/9/15

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N2 - It is demonstrated that biomimetic stable triblock copolymer membrane arrays can be prepared using a scaffold containing 64 apertures of 300 μm diameter each. The membranes were made from a stock solution of block copolymers with decane as a solvent using a new deposition method. By using decane, we avoid low molecular weight solvents such as chloroform and toluene, which are strong protein denaturants. The membranes show a low ionic conductance and a long lifetime at room temperature. Contrast phase microscopy shows the presence of a polymer region delimited by a Plateau-Gibbs border similar to what is observed in black lipid membranes. The ion-channel gramicidin A was successfully incorporated into the membrane in a functional form.

AB - It is demonstrated that biomimetic stable triblock copolymer membrane arrays can be prepared using a scaffold containing 64 apertures of 300 μm diameter each. The membranes were made from a stock solution of block copolymers with decane as a solvent using a new deposition method. By using decane, we avoid low molecular weight solvents such as chloroform and toluene, which are strong protein denaturants. The membranes show a low ionic conductance and a long lifetime at room temperature. Contrast phase microscopy shows the presence of a polymer region delimited by a Plateau-Gibbs border similar to what is observed in black lipid membranes. The ion-channel gramicidin A was successfully incorporated into the membrane in a functional form.

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JO - Langmuir

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Biomimetic triblock copolymer membrane arrays: A stable template for functional membrane proteins

Abstract

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