Biomimetic triblock copolymer membrane arrays: A stable template for functional membrane proteins

Alfredo González-Pérez; Karin B. Stibius; Thomas Vissing; Claus H. Nielsen; Ole G. Mouritsen

doi:10.1021/la902417m

Biomimetic triblock copolymer membrane arrays: A stable template for functional membrane proteins

Alfredo González-Pérez^*, Karin B. Stibius, Thomas Vissing, Claus H. Nielsen, Ole G. Mouritsen

^*Corresponding author af dette arbejde

55 Citationer (Scopus)

Abstract

It is demonstrated that biomimetic stable triblock copolymer membrane arrays can be prepared using a scaffold containing 64 apertures of 300 μm diameter each. The membranes were made from a stock solution of block copolymers with decane as a solvent using a new deposition method. By using decane, we avoid low molecular weight solvents such as chloroform and toluene, which are strong protein denaturants. The membranes show a low ionic conductance and a long lifetime at room temperature. Contrast phase microscopy shows the presence of a polymer region delimited by a Plateau-Gibbs border similar to what is observed in black lipid membranes. The ion-channel gramicidin A was successfully incorporated into the membrane in a functional form.

Originalsprog	Engelsk
Tidsskrift	Langmuir
Vol/bind	25
Udgave nummer	18
Sider (fra-til)	10447-10450
Antal sider	4
ISSN	0743-7463
DOI	https://doi.org/10.1021/la902417m
Status	Udgivet - 15 sep. 2009

Adgang til dokumentet

10.1021/la902417m

Andre filer og links

Link to publication in Scopus

Citationsformater

@article{56a7cd6f6b4f4acbbca2353030ec2638,

title = "Biomimetic triblock copolymer membrane arrays: A stable template for functional membrane proteins",

abstract = "It is demonstrated that biomimetic stable triblock copolymer membrane arrays can be prepared using a scaffold containing 64 apertures of 300 μm diameter each. The membranes were made from a stock solution of block copolymers with decane as a solvent using a new deposition method. By using decane, we avoid low molecular weight solvents such as chloroform and toluene, which are strong protein denaturants. The membranes show a low ionic conductance and a long lifetime at room temperature. Contrast phase microscopy shows the presence of a polymer region delimited by a Plateau-Gibbs border similar to what is observed in black lipid membranes. The ion-channel gramicidin A was successfully incorporated into the membrane in a functional form.",

author = "Alfredo Gonz{\'a}lez-P{\'e}rez and Stibius, {Karin B.} and Thomas Vissing and Nielsen, {Claus H.} and Mouritsen, {Ole G.}",

year = "2009",

month = sep,

day = "15",

doi = "10.1021/la902417m",

language = "English",

volume = "25",

pages = "10447--10450",

journal = "Langmuir",

issn = "0743-7463",

publisher = "American Chemical Society",

number = "18",

}

TY - JOUR

T1 - Biomimetic triblock copolymer membrane arrays

T2 - A stable template for functional membrane proteins

AU - González-Pérez, Alfredo

AU - Stibius, Karin B.

AU - Vissing, Thomas

AU - Nielsen, Claus H.

AU - Mouritsen, Ole G.

PY - 2009/9/15

Y1 - 2009/9/15

N2 - It is demonstrated that biomimetic stable triblock copolymer membrane arrays can be prepared using a scaffold containing 64 apertures of 300 μm diameter each. The membranes were made from a stock solution of block copolymers with decane as a solvent using a new deposition method. By using decane, we avoid low molecular weight solvents such as chloroform and toluene, which are strong protein denaturants. The membranes show a low ionic conductance and a long lifetime at room temperature. Contrast phase microscopy shows the presence of a polymer region delimited by a Plateau-Gibbs border similar to what is observed in black lipid membranes. The ion-channel gramicidin A was successfully incorporated into the membrane in a functional form.

AB - It is demonstrated that biomimetic stable triblock copolymer membrane arrays can be prepared using a scaffold containing 64 apertures of 300 μm diameter each. The membranes were made from a stock solution of block copolymers with decane as a solvent using a new deposition method. By using decane, we avoid low molecular weight solvents such as chloroform and toluene, which are strong protein denaturants. The membranes show a low ionic conductance and a long lifetime at room temperature. Contrast phase microscopy shows the presence of a polymer region delimited by a Plateau-Gibbs border similar to what is observed in black lipid membranes. The ion-channel gramicidin A was successfully incorporated into the membrane in a functional form.

UR - http://www.scopus.com/inward/record.url?scp=70349929812&partnerID=8YFLogxK

U2 - 10.1021/la902417m

DO - 10.1021/la902417m

M3 - Journal article

C2 - 19735126

AN - SCOPUS:70349929812

SN - 0743-7463

VL - 25

SP - 10447

EP - 10450

JO - Langmuir

JF - Langmuir

IS - 18

ER -

Biomimetic triblock copolymer membrane arrays: A stable template for functional membrane proteins

Abstract

Adgang til dokumentet

Andre filer og links

Fingeraftryk

Citationsformater