Biolistic transformation of the obligate plant pathogenic fungus, Erysiphe graminis f.sp. hordei

S.K. Christiansen; S. Knudsen; H. Giese

doi:10.1007/BF00313200

Biolistic transformation of the obligate plant pathogenic fungus, Erysiphe graminis f.sp. hordei

24 Citations (Scopus)

Abstract

Particle gun acceleration appears to be a possible way to transform mycelium cells of obligate plant parasites growing on host surfaces, GUS expression was obtained in E. graminis f.sp. hordei cells after bombardment with the GUS gene under the control of the E. graminis f.sp. hordei beta-tubulin promoter. Three heterologous promoters, one from Aspergillus nidulans and two from Cochliobolus heterostrophus, gave very low or no expression of GUS.

Original language	English
Journal	Current Genetics
Volume	29
Issue number	1
Pages (from-to)	100-102
ISSN	0172-8083
DOIs	https://doi.org/10.1007/BF00313200
Publication status	Published - 1995
Externally published	Yes

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title = "Biolistic transformation of the obligate plant pathogenic fungus, Erysiphe graminis f.sp. hordei",

abstract = "Particle gun acceleration appears to be a possible way to transform mycelium cells of obligate plant parasites growing on host surfaces, GUS expression was obtained in E. graminis f.sp. hordei cells after bombardment with the GUS gene under the control of the E. graminis f.sp. hordei beta-tubulin promoter. Three heterologous promoters, one from Aspergillus nidulans and two from Cochliobolus heterostrophus, gave very low or no expression of GUS.",

author = "S.K. Christiansen and S. Knudsen and H. Giese",

year = "1995",

doi = "10.1007/BF00313200",

language = "English",

volume = "29",

pages = "100--102",

journal = "Current Genetics",

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T1 - Biolistic transformation of the obligate plant pathogenic fungus, Erysiphe graminis f.sp. hordei

AU - Christiansen, S.K.

AU - Knudsen, S.

AU - Giese, H.

PY - 1995

Y1 - 1995

N2 - Particle gun acceleration appears to be a possible way to transform mycelium cells of obligate plant parasites growing on host surfaces, GUS expression was obtained in E. graminis f.sp. hordei cells after bombardment with the GUS gene under the control of the E. graminis f.sp. hordei beta-tubulin promoter. Three heterologous promoters, one from Aspergillus nidulans and two from Cochliobolus heterostrophus, gave very low or no expression of GUS.

AB - Particle gun acceleration appears to be a possible way to transform mycelium cells of obligate plant parasites growing on host surfaces, GUS expression was obtained in E. graminis f.sp. hordei cells after bombardment with the GUS gene under the control of the E. graminis f.sp. hordei beta-tubulin promoter. Three heterologous promoters, one from Aspergillus nidulans and two from Cochliobolus heterostrophus, gave very low or no expression of GUS.

U2 - 10.1007/BF00313200

DO - 10.1007/BF00313200

M3 - Journal article

SN - 0172-8083

VL - 29

SP - 100

EP - 102

JO - Current Genetics

JF - Current Genetics

IS - 1

ER -

Biolistic transformation of the obligate plant pathogenic fungus, Erysiphe graminis f.sp. hordei

Abstract

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