Biolistic transformation of the obligate plant pathogenic fungus, Erysiphe graminis f.sp. hordei

TY - JOUR

T1 - Biolistic transformation of the obligate plant pathogenic fungus, Erysiphe graminis f.sp. hordei

AU - Christiansen, S.K.

AU - Knudsen, S.

AU - Giese, H.

PY - 1995

Y1 - 1995

N2 - Particle gun acceleration appears to be a possible way to transform mycelium cells of obligate plant parasites growing on host surfaces, GUS expression was obtained in E. graminis f.sp. hordei cells after bombardment with the GUS gene under the control of the E. graminis f.sp. hordei beta-tubulin promoter. Three heterologous promoters, one from Aspergillus nidulans and two from Cochliobolus heterostrophus, gave very low or no expression of GUS.

AB - Particle gun acceleration appears to be a possible way to transform mycelium cells of obligate plant parasites growing on host surfaces, GUS expression was obtained in E. graminis f.sp. hordei cells after bombardment with the GUS gene under the control of the E. graminis f.sp. hordei beta-tubulin promoter. Three heterologous promoters, one from Aspergillus nidulans and two from Cochliobolus heterostrophus, gave very low or no expression of GUS.

U2 - 10.1007/BF00313200

DO - 10.1007/BF00313200

M3 - Journal article

SN - 0172-8083

VL - 29

SP - 100

EP - 102

JO - Current Genetics

JF - Current Genetics

IS - 1

ER -