Bioenergetic Changes during Differentiation of Human Embryonic Stem Cells along the Hepatic Lineage

Branden M Hopkinson; Claus Desler Madsen; Mark Kalisz; Peter Siig Vestentoft; Lene Juel Rasmussen; Hanne Cathrine Bisgaard

doi:10.1155/2017/5080128

Bioenergetic Changes during Differentiation of Human Embryonic Stem Cells along the Hepatic Lineage

Branden M Hopkinson, Claus Desler Madsen, Mark Kalisz, Peter Siig Vestentoft, Lene Juel Rasmussen, Hanne Cathrine Bisgaard

11 Citations (Scopus)

132 Downloads (Pure)

Abstract

Mitochondrial dysfunction has been demonstrated to result in premature aging due to its effects on stem cells. Nevertheless, a full understanding of the role of mitochondrial bioenergetics through differentiation is still lacking. Here we show the bioenergetics profile of human stem cells of embryonic origin differentiating along the hepatic lineage. Our study reveals especially the transition between hepatic specification and hepatic maturation as dependent on mitochondrial respiration and demonstrates that even though differentiating cells are primarily dependent on glycolysis until induction of hepatocyte maturation, oxidative phosphorylation is essential at all stages of differentiation.

Original language	English
Article number	5080128
Journal	Oxidative Medicine and Cellular Longevity
Volume	2017
Number of pages	11
ISSN	1942-0900
DOIs	https://doi.org/10.1155/2017/5080128
Publication status	Published - 2017

Keywords

Cell Differentiation
Cell Line
Cell Respiration
Energy Metabolism
Fluorescent Antibody Technique, Indirect
Hepatocytes
Human Embryonic Stem Cells
Humans
Microscopy, Confocal
Mitochondria
Polymerase Chain Reaction
Journal Article

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Bioenergetic Changes during Differentiation of Human Embryonic Stem Cells along the Hepatic LineageFinal published version, 4.89 MBLicence: CC BY

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title = "Bioenergetic Changes during Differentiation of Human Embryonic Stem Cells along the Hepatic Lineage",

abstract = "Mitochondrial dysfunction has been demonstrated to result in premature aging due to its effects on stem cells. Nevertheless, a full understanding of the role of mitochondrial bioenergetics through differentiation is still lacking. Here we show the bioenergetics profile of human stem cells of embryonic origin differentiating along the hepatic lineage. Our study reveals especially the transition between hepatic specification and hepatic maturation as dependent on mitochondrial respiration and demonstrates that even though differentiating cells are primarily dependent on glycolysis until induction of hepatocyte maturation, oxidative phosphorylation is essential at all stages of differentiation.",

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doi = "10.1155/2017/5080128",

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journal = "Oxidative Medicine and Cellular Longevity",

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AU - Hopkinson, Branden M

AU - Madsen, Claus Desler

AU - Kalisz, Mark

AU - Vestentoft, Peter Siig

AU - Juel Rasmussen, Lene

AU - Bisgaard, Hanne Cathrine

PY - 2017

Y1 - 2017

N2 - Mitochondrial dysfunction has been demonstrated to result in premature aging due to its effects on stem cells. Nevertheless, a full understanding of the role of mitochondrial bioenergetics through differentiation is still lacking. Here we show the bioenergetics profile of human stem cells of embryonic origin differentiating along the hepatic lineage. Our study reveals especially the transition between hepatic specification and hepatic maturation as dependent on mitochondrial respiration and demonstrates that even though differentiating cells are primarily dependent on glycolysis until induction of hepatocyte maturation, oxidative phosphorylation is essential at all stages of differentiation.

AB - Mitochondrial dysfunction has been demonstrated to result in premature aging due to its effects on stem cells. Nevertheless, a full understanding of the role of mitochondrial bioenergetics through differentiation is still lacking. Here we show the bioenergetics profile of human stem cells of embryonic origin differentiating along the hepatic lineage. Our study reveals especially the transition between hepatic specification and hepatic maturation as dependent on mitochondrial respiration and demonstrates that even though differentiating cells are primarily dependent on glycolysis until induction of hepatocyte maturation, oxidative phosphorylation is essential at all stages of differentiation.

KW - Cell Differentiation

KW - Cell Line

KW - Cell Respiration

KW - Energy Metabolism

KW - Fluorescent Antibody Technique, Indirect

KW - Hepatocytes

KW - Human Embryonic Stem Cells

KW - Humans

KW - Microscopy, Confocal

KW - Mitochondria

KW - Polymerase Chain Reaction

KW - Journal Article

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DO - 10.1155/2017/5080128

M3 - Journal article

C2 - 28265337

SN - 1942-0900

VL - 2017

JO - Oxidative Medicine and Cellular Longevity

JF - Oxidative Medicine and Cellular Longevity

M1 - 5080128

ER -

Bioenergetic Changes during Differentiation of Human Embryonic Stem Cells along the Hepatic Lineage

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