Authentication and assessment of contamination in ancient DNA

Gabriel Renaud, Mikkel Schubert, Susanna Sawyer, Ludovic Orlando*

*Corresponding author for this work
    3 Citations (Scopus)

    Abstract

    Contamination from both present-day humans and postmortem microbial sources is a common challenge in ancient DNA studies. Here we present a suite of tools to assist in the assessment of contamination in ancient DNA data sets. These tools perform standard tests of authenticity of ancient DNA data including detecting the presence of postmortem damage signatures in sequence alignments and quantifying the amount of present-day human contamination.

    Original languageEnglish
    Title of host publicationAncient DNA : Methods and Protocols
    EditorsBeth Shapiro, Axel Barlow, Peter D. Heintzman, Michael Hofreiter, Johanna L. A. Paijmans, André E. R. Soares
    Number of pages32
    PublisherHumana Press
    Publication date2019
    Edition2. ed.
    Pages163-194
    Chapter17
    ISBN (Print) 978-1-4939-9175-4
    ISBN (Electronic)978-1-4939-9176-1
    DOIs
    Publication statusPublished - 2019
    SeriesMethods in Molecular Biology
    Volume1963
    ISSN1064-3745

    Keywords

    • Ancient DNA
    • Contamination
    • DICE
    • mapDamage2.0
    • Postmortem damage
    • Schmutzi

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