Abstract
Background: Oxidative stress may be important in carcinogenesis and a possible risk factor for breast cancer. The urinary excretion of oxidatively generated biomolecules, such as 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG), represents biomarkers of oxidative stress, reflecting the rate of global damage to DNA in steady state. Methods: In a nested case-control design, we examined associations between urinary excretion of 8-oxodG and risk of breast cancer in a population-based cohort of 24,697 postmenopausal women aged 50 to 64 years with 3 to 7 years follow-up. The accruing cases of breast cancer were matched to controls by age at diagnosis, baseline age, and hormone replacement therapy (HRT). Spot urine samples collected at entry was analyzed for 8-oxodG by high-performance liquid chromatography with electrochemical detection. Incidence rate ratio (IRR; 95% confidence intervals) based on 336 matched pairs with all information was estimated per unit increase in 8-oxodG divided by creatinine for all and estrogen receptor (ER) positive and negative breast cancers. Results: There was a borderline significant positive association between 8-oxodG and risk of all breast cancer (IRR: 1.08; 1.00-1.17 per unit increase in nmol/mmol creatinine). This association was significant with respect to the risk of ER-positive cancer (IRR: 1.11; 1.01-1.23) and among women not using HRT (IRR: 1.11; 0.97-1.26) or with low dietary iron intake (IRR: 1.10; 1.06-1.37 per unit increase) for all breast cancer. Conclusions: We observed positive association between 8-oxodG excretion and risk of especially ER-positive breast cancer. Impact: Our results suggest that oxidative stress with damage to DNA is important for the development of breast cancer.
Original language | English |
---|---|
Journal | Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology |
Volume | 22 |
Issue number | 7 |
Pages (from-to) | 1289-96 |
Number of pages | 8 |
ISSN | 1055-9965 |
DOIs | |
Publication status | Published - Jul 2013 |
Keywords
- Breast Neoplasms
- Case-Control Studies
- Cohort Studies
- DNA Damage
- Denmark
- Deoxyguanosine
- Diet
- Female
- Follow-Up Studies
- Humans
- Middle Aged
- Oxidative Stress
- Postmenopause
- Prospective Studies
- Receptors, Estrogen
- Risk Factors
- Tumor Markers, Biological