Assessment of polycyclic aromatic hydrocarbon exposure, lung function, systemic inflammation, and genotoxicity in peripheral blood mononuclear cells from firefighters before and after a work shift

Maria Helena Guerra Andersen; Anne Thoustrup Saber; Julie Elbæk Pedersen; Peter Bøgh Pedersen; Per Axel Clausen; Mille Løhr; Ali Kermanizadeh; Steffen Loft; Niels Erik Ebbehøj; Åse Marie Hansen; Ismo Kalevi Koponen; Eva-Carina Nørskov; Ulla Vogel; Peter Møller

doi:10.1002/em.22193

Assessment of polycyclic aromatic hydrocarbon exposure, lung function, systemic inflammation, and genotoxicity in peripheral blood mononuclear cells from firefighters before and after a work shift

Maria Helena Guerra Andersen, Anne Thoustrup Saber, Julie Elbæk Pedersen, Peter Bøgh Pedersen, Per Axel Clausen, Mille Løhr, Ali Kermanizadeh, Steffen Loft, Niels Erik Ebbehøj, Åse Marie Hansen, Ismo Kalevi Koponen, Eva-Carina Nørskov, Ulla Vogel, Peter Møller

13 Citations (Scopus)

Abstract

Firefighting is regarded as possibly carcinogenic, although there are few mechanistic studies on genotoxicity in humans. We investigated exposure to polycyclic aromatic hydrocarbons (PAH), lung function, systemic inflammation and genotoxicity in peripheral blood mononuclear cells (PBMC) of 22 professional firefighters before and after a 24-h work shift. Exposure was assessed by measurements of particulate matter (PM), PAH levels on skin, urinary 1-hydroxypyrene (1-OHP) and self-reported participation in fire extinguishing activities. PM measurements indicated that use of personal protective equipment (PPE) effectively prevented inhalation exposure, but exposure to PM occurred when the environment was perceived as safe and the self-contained breathing apparatuses were removed. The level of PAH on skin and urinary 1-OHP concentration were similar before and after the work shift, irrespective of self-reported participation in fire extinction activities. Post-shift, the subjects had reduced levels of oxidatively damaged DNA in PBMC, and increased plasma concentration of vascular cell adhesion molecule 1 (VCAM-1). The subjects reporting participation in fire extinction activities during the work shift had a slightly decreased lung function, increased plasma concentration of VCAM-1, and reduced levels of oxidatively damaged DNA in PBMC. Our results suggest that the firefighters were not exposed to PM while using PPE, but exposure occurred when PPE was not used. The work shift was not associated with increased levels of genotoxicity. Increased levels of VCAM-1 in plasma were observed. Environ. Mol. Mutagen. 59:539–548, 2018.

Original language	English
Journal	Environmental and Molecular Mutagenesis
Volume	59
Issue number	6
Pages (from-to)	539-548
Number of pages	10
ISSN	0893-6692
DOIs	https://doi.org/10.1002/em.22193
Publication status	Published - Jul 2018

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Andersen, M. H. G., Saber, A. T., Pedersen, J. E., Pedersen, P. B., Clausen, P. A., Løhr, M., Kermanizadeh, A., Loft, S., Ebbehøj, N. E., Hansen, Å. M., Kalevi Koponen, I., Nørskov, E.-C., Vogel, U., & Møller, P. (2018). Assessment of polycyclic aromatic hydrocarbon exposure, lung function, systemic inflammation, and genotoxicity in peripheral blood mononuclear cells from firefighters before and after a work shift. Environmental and Molecular Mutagenesis, 59(6), 539-548. https://doi.org/10.1002/em.22193

Assessment of polycyclic aromatic hydrocarbon exposure, lung function, systemic inflammation, and genotoxicity in peripheral blood mononuclear cells from firefighters before and after a work shift. / Andersen, Maria Helena Guerra; Saber, Anne Thoustrup; Pedersen, Julie Elbæk et al.
In: Environmental and Molecular Mutagenesis, Vol. 59, No. 6, 07.2018, p. 539-548.

Research output: Contribution to journal › Journal article › Research › peer-review

Andersen, MHG, Saber, AT, Pedersen, JE, Pedersen, PB, Clausen, PA, Løhr, M, Kermanizadeh, A, Loft, S, Ebbehøj, NE, Hansen, ÅM, Kalevi Koponen, I, Nørskov, E-C, Vogel, U & Møller, P 2018, 'Assessment of polycyclic aromatic hydrocarbon exposure, lung function, systemic inflammation, and genotoxicity in peripheral blood mononuclear cells from firefighters before and after a work shift', Environmental and Molecular Mutagenesis, vol. 59, no. 6, pp. 539-548. https://doi.org/10.1002/em.22193

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title = "Assessment of polycyclic aromatic hydrocarbon exposure, lung function, systemic inflammation, and genotoxicity in peripheral blood mononuclear cells from firefighters before and after a work shift",

abstract = "Firefighting is regarded as possibly carcinogenic, although there are few mechanistic studies on genotoxicity in humans. We investigated exposure to polycyclic aromatic hydrocarbons (PAH), lung function, systemic inflammation and genotoxicity in peripheral blood mononuclear cells (PBMC) of 22 professional firefighters before and after a 24-h work shift. Exposure was assessed by measurements of particulate matter (PM), PAH levels on skin, urinary 1-hydroxypyrene (1-OHP) and self-reported participation in fire extinguishing activities. PM measurements indicated that use of personal protective equipment (PPE) effectively prevented inhalation exposure, but exposure to PM occurred when the environment was perceived as safe and the self-contained breathing apparatuses were removed. The level of PAH on skin and urinary 1-OHP concentration were similar before and after the work shift, irrespective of self-reported participation in fire extinction activities. Post-shift, the subjects had reduced levels of oxidatively damaged DNA in PBMC, and increased plasma concentration of vascular cell adhesion molecule 1 (VCAM-1). The subjects reporting participation in fire extinction activities during the work shift had a slightly decreased lung function, increased plasma concentration of VCAM-1, and reduced levels of oxidatively damaged DNA in PBMC. Our results suggest that the firefighters were not exposed to PM while using PPE, but exposure occurred when PPE was not used. The work shift was not associated with increased levels of genotoxicity. Increased levels of VCAM-1 in plasma were observed. Environ. Mol. Mutagen. 59:539–548, 2018.",

author = "Andersen, {Maria Helena Guerra} and Saber, {Anne Thoustrup} and Pedersen, {Julie Elb{\ae}k} and Pedersen, {Peter B{\o}gh} and Clausen, {Per Axel} and Mille L{\o}hr and Ali Kermanizadeh and Steffen Loft and Ebbeh{\o}j, {Niels Erik} and Hansen, {{\AA}se Marie} and {Kalevi Koponen}, Ismo and Eva-Carina N{\o}rskov and Ulla Vogel and Peter M{\o}ller",

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T1 - Assessment of polycyclic aromatic hydrocarbon exposure, lung function, systemic inflammation, and genotoxicity in peripheral blood mononuclear cells from firefighters before and after a work shift

AU - Andersen, Maria Helena Guerra

AU - Saber, Anne Thoustrup

AU - Pedersen, Julie Elbæk

AU - Pedersen, Peter Bøgh

AU - Clausen, Per Axel

AU - Løhr, Mille

AU - Kermanizadeh, Ali

AU - Loft, Steffen

AU - Ebbehøj, Niels Erik

AU - Hansen, Åse Marie

AU - Kalevi Koponen, Ismo

AU - Nørskov, Eva-Carina

AU - Vogel, Ulla

AU - Møller, Peter

PY - 2018/7

Y1 - 2018/7

N2 - Firefighting is regarded as possibly carcinogenic, although there are few mechanistic studies on genotoxicity in humans. We investigated exposure to polycyclic aromatic hydrocarbons (PAH), lung function, systemic inflammation and genotoxicity in peripheral blood mononuclear cells (PBMC) of 22 professional firefighters before and after a 24-h work shift. Exposure was assessed by measurements of particulate matter (PM), PAH levels on skin, urinary 1-hydroxypyrene (1-OHP) and self-reported participation in fire extinguishing activities. PM measurements indicated that use of personal protective equipment (PPE) effectively prevented inhalation exposure, but exposure to PM occurred when the environment was perceived as safe and the self-contained breathing apparatuses were removed. The level of PAH on skin and urinary 1-OHP concentration were similar before and after the work shift, irrespective of self-reported participation in fire extinction activities. Post-shift, the subjects had reduced levels of oxidatively damaged DNA in PBMC, and increased plasma concentration of vascular cell adhesion molecule 1 (VCAM-1). The subjects reporting participation in fire extinction activities during the work shift had a slightly decreased lung function, increased plasma concentration of VCAM-1, and reduced levels of oxidatively damaged DNA in PBMC. Our results suggest that the firefighters were not exposed to PM while using PPE, but exposure occurred when PPE was not used. The work shift was not associated with increased levels of genotoxicity. Increased levels of VCAM-1 in plasma were observed. Environ. Mol. Mutagen. 59:539–548, 2018.

AB - Firefighting is regarded as possibly carcinogenic, although there are few mechanistic studies on genotoxicity in humans. We investigated exposure to polycyclic aromatic hydrocarbons (PAH), lung function, systemic inflammation and genotoxicity in peripheral blood mononuclear cells (PBMC) of 22 professional firefighters before and after a 24-h work shift. Exposure was assessed by measurements of particulate matter (PM), PAH levels on skin, urinary 1-hydroxypyrene (1-OHP) and self-reported participation in fire extinguishing activities. PM measurements indicated that use of personal protective equipment (PPE) effectively prevented inhalation exposure, but exposure to PM occurred when the environment was perceived as safe and the self-contained breathing apparatuses were removed. The level of PAH on skin and urinary 1-OHP concentration were similar before and after the work shift, irrespective of self-reported participation in fire extinction activities. Post-shift, the subjects had reduced levels of oxidatively damaged DNA in PBMC, and increased plasma concentration of vascular cell adhesion molecule 1 (VCAM-1). The subjects reporting participation in fire extinction activities during the work shift had a slightly decreased lung function, increased plasma concentration of VCAM-1, and reduced levels of oxidatively damaged DNA in PBMC. Our results suggest that the firefighters were not exposed to PM while using PPE, but exposure occurred when PPE was not used. The work shift was not associated with increased levels of genotoxicity. Increased levels of VCAM-1 in plasma were observed. Environ. Mol. Mutagen. 59:539–548, 2018.

U2 - 10.1002/em.22193

DO - 10.1002/em.22193

M3 - Journal article

C2 - 29761929

SN - 0893-6692

VL - 59

SP - 539

EP - 548

JO - Environmental and Molecular Mutagenesis

JF - Environmental and Molecular Mutagenesis

IS - 6

ER -

Assessment of polycyclic aromatic hydrocarbon exposure, lung function, systemic inflammation, and genotoxicity in peripheral blood mononuclear cells from firefighters before and after a work shift

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