Assessment of flhDC mRNA levels in Serratia liquefaciens swarm cells.

Tim Tolker-Nielsen; A B Christensen; K Holmstrøm; L Eberl; T B Rasmussen; C Sternberg; A Heydorn; S Molin; Michael Givskov

Assessment of flhDC mRNA levels in Serratia liquefaciens swarm cells.

Tim Tolker-Nielsen, A B Christensen, K Holmstrøm, L Eberl, T B Rasmussen, C Sternberg, A Heydorn, S Molin, Michael Givskov

11 Citations (Scopus)

Abstract

We reported previously that artificial overexpression of the flhDC operon in liquid-grown Serratia liquefaciens resulted in the formation of filamentous, multinucleated, and hyperflagellated cells that were indistinguishable from surface-induced swarm cells (L. Eberl, G. Christiansen, S. Molin, and M. Givskov, J. Bacteriol. 178:554-559, 1996). In the present report we show by means of reporter gene measurements, Northern analysis, and in situ reverse transcription-PCR that the amount of flhDC mRNA in surface-grown swarm cells does not exceed the maximum level found in nondifferentiated, vegetative cells. This suggests that surface-induced S. liquefaciens swarm cell differentiation, although dependent on flhDC gene expression, does not occur through elevated flhDC mRNA levels.

Original language	English
Journal	Journal of Bacteriology
Volume	182
Issue number	10
Pages (from-to)	2680-6
Number of pages	6
ISSN	0021-9193
Publication status	Published - 2000
Externally published	Yes

Cite this

@article{020e5d90bd4211dd8e02000ea68e967b,

title = "Assessment of flhDC mRNA levels in Serratia liquefaciens swarm cells.",

abstract = "We reported previously that artificial overexpression of the flhDC operon in liquid-grown Serratia liquefaciens resulted in the formation of filamentous, multinucleated, and hyperflagellated cells that were indistinguishable from surface-induced swarm cells (L. Eberl, G. Christiansen, S. Molin, and M. Givskov, J. Bacteriol. 178:554-559, 1996). In the present report we show by means of reporter gene measurements, Northern analysis, and in situ reverse transcription-PCR that the amount of flhDC mRNA in surface-grown swarm cells does not exceed the maximum level found in nondifferentiated, vegetative cells. This suggests that surface-induced S. liquefaciens swarm cell differentiation, although dependent on flhDC gene expression, does not occur through elevated flhDC mRNA levels.",

author = "Tim Tolker-Nielsen and Christensen, {A B} and K Holmstr{\o}m and L Eberl and Rasmussen, {T B} and C Sternberg and A Heydorn and S Molin and Michael Givskov",

note = "Keywords: Bacterial Proteins; DNA-Binding Proteins; Escherichia coli Proteins; Gene Expression; Gene Expression Regulation, Bacterial; Isopropyl Thiogalactoside; Operon; RNA, Bacterial; RNA, Messenger; Recombinant Fusion Proteins; Serratia; Trans-Activators",

year = "2000",

language = "English",

volume = "182",

pages = "2680--6",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

number = "10",

}

TY - JOUR

T1 - Assessment of flhDC mRNA levels in Serratia liquefaciens swarm cells.

AU - Tolker-Nielsen, Tim

AU - Christensen, A B

AU - Holmstrøm, K

AU - Eberl, L

AU - Rasmussen, T B

AU - Sternberg, C

AU - Heydorn, A

AU - Molin, S

AU - Givskov, Michael

N1 - Keywords: Bacterial Proteins; DNA-Binding Proteins; Escherichia coli Proteins; Gene Expression; Gene Expression Regulation, Bacterial; Isopropyl Thiogalactoside; Operon; RNA, Bacterial; RNA, Messenger; Recombinant Fusion Proteins; Serratia; Trans-Activators

PY - 2000

Y1 - 2000

N2 - We reported previously that artificial overexpression of the flhDC operon in liquid-grown Serratia liquefaciens resulted in the formation of filamentous, multinucleated, and hyperflagellated cells that were indistinguishable from surface-induced swarm cells (L. Eberl, G. Christiansen, S. Molin, and M. Givskov, J. Bacteriol. 178:554-559, 1996). In the present report we show by means of reporter gene measurements, Northern analysis, and in situ reverse transcription-PCR that the amount of flhDC mRNA in surface-grown swarm cells does not exceed the maximum level found in nondifferentiated, vegetative cells. This suggests that surface-induced S. liquefaciens swarm cell differentiation, although dependent on flhDC gene expression, does not occur through elevated flhDC mRNA levels.

AB - We reported previously that artificial overexpression of the flhDC operon in liquid-grown Serratia liquefaciens resulted in the formation of filamentous, multinucleated, and hyperflagellated cells that were indistinguishable from surface-induced swarm cells (L. Eberl, G. Christiansen, S. Molin, and M. Givskov, J. Bacteriol. 178:554-559, 1996). In the present report we show by means of reporter gene measurements, Northern analysis, and in situ reverse transcription-PCR that the amount of flhDC mRNA in surface-grown swarm cells does not exceed the maximum level found in nondifferentiated, vegetative cells. This suggests that surface-induced S. liquefaciens swarm cell differentiation, although dependent on flhDC gene expression, does not occur through elevated flhDC mRNA levels.

M3 - Journal article

C2 - 10781533

SN - 0021-9193

VL - 182

SP - 2680

EP - 2686

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 10

ER -

Assessment of flhDC mRNA levels in Serratia liquefaciens swarm cells.

Abstract

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