Aspen pectate lyase PtxtPL1-27 mobilizes matrix polysaccharides from woody tissues and improves saccharification yield

Ajaya K. Biswal; Kazuo Soeno; Madhavi Latha Gandla; Peter Immerzeel; Sivakumar Pattathil; Jessica Lucenius; Ritva Serimaa; Michael G. Hahn; Thomas Moritz; Leif J. Jonsson; Maria Israelsson-Nordstrom; Ewa J. Mellerowicz

doi:10.1186/1754-6834-7-11

Aspen pectate lyase PtxtPL1-27 mobilizes matrix polysaccharides from woody tissues and improves saccharification yield

Ajaya K. Biswal, Kazuo Soeno, Madhavi Latha Gandla, Peter Immerzeel, Sivakumar Pattathil, Jessica Lucenius, Ritva Serimaa, Michael G. Hahn, Thomas Moritz, Leif J. Jonsson, Maria Israelsson-Nordstrom, Ewa J. Mellerowicz

51 Citations (Scopus)

Abstract

Background: Wood cell walls are rich in cellulose, hemicellulose and lignin. Hence, they are important sources of renewable biomass for producing energy and green chemicals. However, extracting desired constituents from wood efficiently poses significant challenges because these polymers are highly cross-linked in cell walls and are not easily accessible to enzymes and chemicals. Results: We show that aspen pectate lyase PL1-27, which degrades homogalacturonan and is expressed at the onset of secondary wall formation, can increase the solubility of wood matrix polysaccharides. Overexpression of this enzyme in aspen increased solubility of not only pectins but also xylans and other hemicelluloses, indicating that homogalacturonan limits the solubility of major wood cell wall components. Enzymatic saccharification of wood obtained from PL1-27-overexpressing trees gave higher yields of pentoses and hexoses than similar treatment of wood from wild-type trees, even after acid pretreatment. Conclusions: Thus, the modification of pectins may constitute an important biotechnological target for improved wood processing despite their low abundance in woody biomass.

Original language	English
Journal	Biotechnology for Biofuels
Volume	7
ISSN	1754-6834
DOIs	https://doi.org/10.1186/1754-6834-7-11
Publication status	Published - 22 Jan 2014
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1186/1754-6834-7-11Licence: CC BY

1754-6834-7-11Final published version, 1.51 MBLicence: CC BY

Cite this

Biswal, A. K., Soeno, K., Gandla, M. L., Immerzeel, P., Pattathil, S., Lucenius, J., Serimaa, R., Hahn, M. G., Moritz, T., Jonsson, L. J., Israelsson-Nordstrom, M., & Mellerowicz, E. J. (2014). Aspen pectate lyase PtxtPL1-27 mobilizes matrix polysaccharides from woody tissues and improves saccharification yield. Biotechnology for Biofuels, 7. https://doi.org/10.1186/1754-6834-7-11

@article{7460e57322f842639afe6bfbe234c50d,

title = "Aspen pectate lyase PtxtPL1-27 mobilizes matrix polysaccharides from woody tissues and improves saccharification yield",

abstract = "Background: Wood cell walls are rich in cellulose, hemicellulose and lignin. Hence, they are important sources of renewable biomass for producing energy and green chemicals. However, extracting desired constituents from wood efficiently poses significant challenges because these polymers are highly cross-linked in cell walls and are not easily accessible to enzymes and chemicals. Results: We show that aspen pectate lyase PL1-27, which degrades homogalacturonan and is expressed at the onset of secondary wall formation, can increase the solubility of wood matrix polysaccharides. Overexpression of this enzyme in aspen increased solubility of not only pectins but also xylans and other hemicelluloses, indicating that homogalacturonan limits the solubility of major wood cell wall components. Enzymatic saccharification of wood obtained from PL1-27-overexpressing trees gave higher yields of pentoses and hexoses than similar treatment of wood from wild-type trees, even after acid pretreatment. Conclusions: Thus, the modification of pectins may constitute an important biotechnological target for improved wood processing despite their low abundance in woody biomass.",

author = "Biswal, {Ajaya K.} and Kazuo Soeno and Gandla, {Madhavi Latha} and Peter Immerzeel and Sivakumar Pattathil and Jessica Lucenius and Ritva Serimaa and Hahn, {Michael G.} and Thomas Moritz and Jonsson, {Leif J.} and Maria Israelsson-Nordstrom and Mellerowicz, {Ewa J.}",

year = "2014",

month = jan,

day = "22",

doi = "10.1186/1754-6834-7-11",

language = "English",

volume = "7",

journal = "Biotechnology for Biofuels",

issn = "1754-6834",

publisher = "BioMed Central Ltd.",

}

TY - JOUR

T1 - Aspen pectate lyase PtxtPL1-27 mobilizes matrix polysaccharides from woody tissues and improves saccharification yield

AU - Biswal, Ajaya K.

AU - Soeno, Kazuo

AU - Gandla, Madhavi Latha

AU - Immerzeel, Peter

AU - Pattathil, Sivakumar

AU - Lucenius, Jessica

AU - Serimaa, Ritva

AU - Hahn, Michael G.

AU - Moritz, Thomas

AU - Jonsson, Leif J.

AU - Israelsson-Nordstrom, Maria

AU - Mellerowicz, Ewa J.

PY - 2014/1/22

Y1 - 2014/1/22

N2 - Background: Wood cell walls are rich in cellulose, hemicellulose and lignin. Hence, they are important sources of renewable biomass for producing energy and green chemicals. However, extracting desired constituents from wood efficiently poses significant challenges because these polymers are highly cross-linked in cell walls and are not easily accessible to enzymes and chemicals. Results: We show that aspen pectate lyase PL1-27, which degrades homogalacturonan and is expressed at the onset of secondary wall formation, can increase the solubility of wood matrix polysaccharides. Overexpression of this enzyme in aspen increased solubility of not only pectins but also xylans and other hemicelluloses, indicating that homogalacturonan limits the solubility of major wood cell wall components. Enzymatic saccharification of wood obtained from PL1-27-overexpressing trees gave higher yields of pentoses and hexoses than similar treatment of wood from wild-type trees, even after acid pretreatment. Conclusions: Thus, the modification of pectins may constitute an important biotechnological target for improved wood processing despite their low abundance in woody biomass.

AB - Background: Wood cell walls are rich in cellulose, hemicellulose and lignin. Hence, they are important sources of renewable biomass for producing energy and green chemicals. However, extracting desired constituents from wood efficiently poses significant challenges because these polymers are highly cross-linked in cell walls and are not easily accessible to enzymes and chemicals. Results: We show that aspen pectate lyase PL1-27, which degrades homogalacturonan and is expressed at the onset of secondary wall formation, can increase the solubility of wood matrix polysaccharides. Overexpression of this enzyme in aspen increased solubility of not only pectins but also xylans and other hemicelluloses, indicating that homogalacturonan limits the solubility of major wood cell wall components. Enzymatic saccharification of wood obtained from PL1-27-overexpressing trees gave higher yields of pentoses and hexoses than similar treatment of wood from wild-type trees, even after acid pretreatment. Conclusions: Thus, the modification of pectins may constitute an important biotechnological target for improved wood processing despite their low abundance in woody biomass.

U2 - 10.1186/1754-6834-7-11

DO - 10.1186/1754-6834-7-11

M3 - Journal article

SN - 1754-6834

VL - 7

JO - Biotechnology for Biofuels

JF - Biotechnology for Biofuels

ER -

Aspen pectate lyase PtxtPL1-27 mobilizes matrix polysaccharides from woody tissues and improves saccharification yield

Abstract

UN SDGs

Access to Document

Fingerprint

Cite this