Aspen pectate lyase PtxtPL1-27 mobilizes matrix polysaccharides from woody tissues and improves saccharification yield

Ajaya K. Biswal; Kazuo Soeno; Madhavi Latha Gandla; Peter Immerzeel; Sivakumar Pattathil; Jessica Lucenius; Ritva Serimaa; Michael G. Hahn; Thomas Moritz; Leif J. Jonsson; Maria Israelsson-Nordstrom; Ewa J. Mellerowicz

doi:10.1186/1754-6834-7-11

Aspen pectate lyase PtxtPL1-27 mobilizes matrix polysaccharides from woody tissues and improves saccharification yield

Ajaya K. Biswal, Kazuo Soeno, Madhavi Latha Gandla, Peter Immerzeel, Sivakumar Pattathil, Jessica Lucenius, Ritva Serimaa, Michael G. Hahn, Thomas Moritz, Leif J. Jonsson, Maria Israelsson-Nordstrom, Ewa J. Mellerowicz

51 Citationer (Scopus)

Abstract

Background: Wood cell walls are rich in cellulose, hemicellulose and lignin. Hence, they are important sources of renewable biomass for producing energy and green chemicals. However, extracting desired constituents from wood efficiently poses significant challenges because these polymers are highly cross-linked in cell walls and are not easily accessible to enzymes and chemicals. Results: We show that aspen pectate lyase PL1-27, which degrades homogalacturonan and is expressed at the onset of secondary wall formation, can increase the solubility of wood matrix polysaccharides. Overexpression of this enzyme in aspen increased solubility of not only pectins but also xylans and other hemicelluloses, indicating that homogalacturonan limits the solubility of major wood cell wall components. Enzymatic saccharification of wood obtained from PL1-27-overexpressing trees gave higher yields of pentoses and hexoses than similar treatment of wood from wild-type trees, even after acid pretreatment. Conclusions: Thus, the modification of pectins may constitute an important biotechnological target for improved wood processing despite their low abundance in woody biomass.

Originalsprog	Engelsk
Tidsskrift	Biotechnology for Biofuels
Vol/bind	7
ISSN	1754-6834
DOI	https://doi.org/10.1186/1754-6834-7-11
Status	Udgivet - 22 jan. 2014
Udgivet eksternt	Ja

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10.1186/1754-6834-7-11Licens: CC BY

1754-6834-7-11Forlagets udgivne version, 1,51 MBLicens: CC BY

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Biswal, A. K., Soeno, K., Gandla, M. L., Immerzeel, P., Pattathil, S., Lucenius, J., Serimaa, R., Hahn, M. G., Moritz, T., Jonsson, L. J., Israelsson-Nordstrom, M., & Mellerowicz, E. J. (2014). Aspen pectate lyase PtxtPL1-27 mobilizes matrix polysaccharides from woody tissues and improves saccharification yield. Biotechnology for Biofuels, 7. https://doi.org/10.1186/1754-6834-7-11

@article{7460e57322f842639afe6bfbe234c50d,

title = "Aspen pectate lyase PtxtPL1-27 mobilizes matrix polysaccharides from woody tissues and improves saccharification yield",

abstract = "Background: Wood cell walls are rich in cellulose, hemicellulose and lignin. Hence, they are important sources of renewable biomass for producing energy and green chemicals. However, extracting desired constituents from wood efficiently poses significant challenges because these polymers are highly cross-linked in cell walls and are not easily accessible to enzymes and chemicals. Results: We show that aspen pectate lyase PL1-27, which degrades homogalacturonan and is expressed at the onset of secondary wall formation, can increase the solubility of wood matrix polysaccharides. Overexpression of this enzyme in aspen increased solubility of not only pectins but also xylans and other hemicelluloses, indicating that homogalacturonan limits the solubility of major wood cell wall components. Enzymatic saccharification of wood obtained from PL1-27-overexpressing trees gave higher yields of pentoses and hexoses than similar treatment of wood from wild-type trees, even after acid pretreatment. Conclusions: Thus, the modification of pectins may constitute an important biotechnological target for improved wood processing despite their low abundance in woody biomass.",

author = "Biswal, {Ajaya K.} and Kazuo Soeno and Gandla, {Madhavi Latha} and Peter Immerzeel and Sivakumar Pattathil and Jessica Lucenius and Ritva Serimaa and Hahn, {Michael G.} and Thomas Moritz and Jonsson, {Leif J.} and Maria Israelsson-Nordstrom and Mellerowicz, {Ewa J.}",

year = "2014",

month = jan,

day = "22",

doi = "10.1186/1754-6834-7-11",

language = "English",

volume = "7",

journal = "Biotechnology for Biofuels",

issn = "1754-6834",

publisher = "BioMed Central Ltd.",

}

TY - JOUR

T1 - Aspen pectate lyase PtxtPL1-27 mobilizes matrix polysaccharides from woody tissues and improves saccharification yield

AU - Biswal, Ajaya K.

AU - Soeno, Kazuo

AU - Gandla, Madhavi Latha

AU - Immerzeel, Peter

AU - Pattathil, Sivakumar

AU - Lucenius, Jessica

AU - Serimaa, Ritva

AU - Hahn, Michael G.

AU - Moritz, Thomas

AU - Jonsson, Leif J.

AU - Israelsson-Nordstrom, Maria

AU - Mellerowicz, Ewa J.

PY - 2014/1/22

Y1 - 2014/1/22

N2 - Background: Wood cell walls are rich in cellulose, hemicellulose and lignin. Hence, they are important sources of renewable biomass for producing energy and green chemicals. However, extracting desired constituents from wood efficiently poses significant challenges because these polymers are highly cross-linked in cell walls and are not easily accessible to enzymes and chemicals. Results: We show that aspen pectate lyase PL1-27, which degrades homogalacturonan and is expressed at the onset of secondary wall formation, can increase the solubility of wood matrix polysaccharides. Overexpression of this enzyme in aspen increased solubility of not only pectins but also xylans and other hemicelluloses, indicating that homogalacturonan limits the solubility of major wood cell wall components. Enzymatic saccharification of wood obtained from PL1-27-overexpressing trees gave higher yields of pentoses and hexoses than similar treatment of wood from wild-type trees, even after acid pretreatment. Conclusions: Thus, the modification of pectins may constitute an important biotechnological target for improved wood processing despite their low abundance in woody biomass.

AB - Background: Wood cell walls are rich in cellulose, hemicellulose and lignin. Hence, they are important sources of renewable biomass for producing energy and green chemicals. However, extracting desired constituents from wood efficiently poses significant challenges because these polymers are highly cross-linked in cell walls and are not easily accessible to enzymes and chemicals. Results: We show that aspen pectate lyase PL1-27, which degrades homogalacturonan and is expressed at the onset of secondary wall formation, can increase the solubility of wood matrix polysaccharides. Overexpression of this enzyme in aspen increased solubility of not only pectins but also xylans and other hemicelluloses, indicating that homogalacturonan limits the solubility of major wood cell wall components. Enzymatic saccharification of wood obtained from PL1-27-overexpressing trees gave higher yields of pentoses and hexoses than similar treatment of wood from wild-type trees, even after acid pretreatment. Conclusions: Thus, the modification of pectins may constitute an important biotechnological target for improved wood processing despite their low abundance in woody biomass.

U2 - 10.1186/1754-6834-7-11

DO - 10.1186/1754-6834-7-11

M3 - Journal article

SN - 1754-6834

VL - 7

JO - Biotechnology for Biofuels

JF - Biotechnology for Biofuels

ER -

Aspen pectate lyase PtxtPL1-27 mobilizes matrix polysaccharides from woody tissues and improves saccharification yield

Abstract

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