Aspects of the barley seed proteome during development and germination

C Finnie; Kenji Maeda; O ØStergaard; Kristian Sass Bak-Jensen; J Larsen; B Svensson

doi:10.1042/BST0320517

Aspects of the barley seed proteome during development and germination

C Finnie, Kenji Maeda, O ØStergaard, Kristian Sass Bak-Jensen, J Larsen, B Svensson

Department of Chemistry

57 Citations (Scopus)

Abstract

Analysis of the water-soluble barley seed proteome has led to the identification of proteins by MS in the major spots on two-dimensional gels covering the pI ranges 4-7 and 6-11. This provides the basis for in-depth studies of proteome changes during seed development and germination, tissue-specific proteomes, cultivar differences related to quality parameters, analysis of the genetic basis for spot variations and targeted investigations of specific proteins.

Original language	English
Journal	Biochemical Society Transactions
Volume	32
Issue number	Pt3
Pages (from-to)	517-9
Number of pages	3
ISSN	0300-5127
DOIs	https://doi.org/10.1042/BST0320517
Publication status	Published - Jun 2004

Keywords

Electrophoresis, Gel, Two-Dimensional
Germination
Hordeum/metabolism
Hydrogen-Ion Concentration
Mass Spectrometry
Plant Proteins/chemistry
Proteome
Seeds/metabolism
Species Specificity
Water/chemistry

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10.1042/BST0320517

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title = "Aspects of the barley seed proteome during development and germination",

abstract = "Analysis of the water-soluble barley seed proteome has led to the identification of proteins by MS in the major spots on two-dimensional gels covering the pI ranges 4-7 and 6-11. This provides the basis for in-depth studies of proteome changes during seed development and germination, tissue-specific proteomes, cultivar differences related to quality parameters, analysis of the genetic basis for spot variations and targeted investigations of specific proteins.",

keywords = "Electrophoresis, Gel, Two-Dimensional, Germination, Hordeum/metabolism, Hydrogen-Ion Concentration, Mass Spectrometry, Plant Proteins/chemistry, Proteome, Seeds/metabolism, Species Specificity, Water/chemistry",

author = "C Finnie and Kenji Maeda and O {\O}Stergaard and Bak-Jensen, {Kristian Sass} and J Larsen and B Svensson",

year = "2004",

month = jun,

doi = "10.1042/BST0320517",

language = "English",

volume = "32",

pages = "517--9",

journal = "Biochemical Society Transactions",

issn = "0300-5127",

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TY - JOUR

T1 - Aspects of the barley seed proteome during development and germination

AU - Finnie, C

AU - Maeda, Kenji

AU - ØStergaard, O

AU - Bak-Jensen, Kristian Sass

AU - Larsen, J

AU - Svensson, B

PY - 2004/6

Y1 - 2004/6

N2 - Analysis of the water-soluble barley seed proteome has led to the identification of proteins by MS in the major spots on two-dimensional gels covering the pI ranges 4-7 and 6-11. This provides the basis for in-depth studies of proteome changes during seed development and germination, tissue-specific proteomes, cultivar differences related to quality parameters, analysis of the genetic basis for spot variations and targeted investigations of specific proteins.

AB - Analysis of the water-soluble barley seed proteome has led to the identification of proteins by MS in the major spots on two-dimensional gels covering the pI ranges 4-7 and 6-11. This provides the basis for in-depth studies of proteome changes during seed development and germination, tissue-specific proteomes, cultivar differences related to quality parameters, analysis of the genetic basis for spot variations and targeted investigations of specific proteins.

KW - Electrophoresis, Gel, Two-Dimensional

KW - Germination

KW - Hordeum/metabolism

KW - Hydrogen-Ion Concentration

KW - Mass Spectrometry

KW - Plant Proteins/chemistry

KW - Proteome

KW - Seeds/metabolism

KW - Species Specificity

KW - Water/chemistry

U2 - 10.1042/BST0320517

DO - 10.1042/BST0320517

M3 - Review

C2 - 15157175

SN - 0300-5127

VL - 32

SP - 517

EP - 519

JO - Biochemical Society Transactions

JF - Biochemical Society Transactions

IS - Pt3

ER -

Aspects of the barley seed proteome during development and germination

Abstract

Keywords

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