Ascorbate and dehydroascorbic acid as reliable biomarkers of oxidative stress: analytical reproducibility and long-term stability of plasma samples subjected to acidic deproteinization

Jens Lykkesfeldt

doi:10.1158/1055-9965.EPI-07-0639

Ascorbate and dehydroascorbic acid as reliable biomarkers of oxidative stress: analytical reproducibility and long-term stability of plasma samples subjected to acidic deproteinization

Jens Lykkesfeldt

70 Citations (Scopus)

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Abstract

Lack of post-sampling stability of ascorbate and dehydroascorbic acid and failure to block their in vivo equilibrium have lowered their value as biomarkers of oxidative stress and limited the ability to further investigate their possible role in disease prevention. In the present paper, the analytical reproducibility was tested by repeated analysis of plasma aliquots from one individual over four years. The plasma was subjected to acidic deproteinization with an equal volume of 10% meta-phosphoric acid containing 2 mM EDTA and analyzed for ascorbate and dehydroascorbic acid by high-performance liquid chromatography with coulometric detection. In a parallel experiment, stability of human plasma samples treated as above and stored at -80°C for five years was tested in a cohort of 131 individuals. No degradation or shift in the equilibrium between ascorbate and dehydroascorbic acid was observed in either of the experiments. In conclusion, ascorbate and dehydroascorbic acid can be adequately preserved in plasma stored at -80°C following acidic deproteinization with meta-phosphoric acid containing 2 mM EDTA.

Original language	English
Journal	Cancer Epidemiology, Biomarkers & Prevention
Volume	16
Issue number	11
Pages (from-to)	2513-2516
Number of pages	4
ISSN	1055-9965
DOIs	https://doi.org/10.1158/1055-9965.EPI-07-0639
Publication status	Published - 2007

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Ascorbate and dehydroascorbic acid as reliable biomarkers of oxidative stress : analytical reproducibility and long-term stability of plasma samples subjected to acidic deproteinization. / Lykkesfeldt, Jens.

In: Cancer Epidemiology, Biomarkers & Prevention, Vol. 16, No. 11, 2007, p. 2513-2516.

Research output: Contribution to journal › Journal article › Research › peer-review

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abstract = "Lack of post-sampling stability of ascorbate and dehydroascorbic acid and failure to block their in vivo equilibrium have lowered their value as biomarkers of oxidative stress and limited the ability to further investigate their possible role in disease prevention. In the present paper, the analytical reproducibility was tested by repeated analysis of plasma aliquots from one individual over four years. The plasma was subjected to acidic deproteinization with an equal volume of 10% meta-phosphoric acid containing 2 mM EDTA and analyzed for ascorbate and dehydroascorbic acid by high-performance liquid chromatography with coulometric detection. In a parallel experiment, stability of human plasma samples treated as above and stored at -80°C for five years was tested in a cohort of 131 individuals. No degradation or shift in the equilibrium between ascorbate and dehydroascorbic acid was observed in either of the experiments. In conclusion, ascorbate and dehydroascorbic acid can be adequately preserved in plasma stored at -80°C following acidic deproteinization with meta-phosphoric acid containing 2 mM EDTA.",

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AB - Lack of post-sampling stability of ascorbate and dehydroascorbic acid and failure to block their in vivo equilibrium have lowered their value as biomarkers of oxidative stress and limited the ability to further investigate their possible role in disease prevention. In the present paper, the analytical reproducibility was tested by repeated analysis of plasma aliquots from one individual over four years. The plasma was subjected to acidic deproteinization with an equal volume of 10% meta-phosphoric acid containing 2 mM EDTA and analyzed for ascorbate and dehydroascorbic acid by high-performance liquid chromatography with coulometric detection. In a parallel experiment, stability of human plasma samples treated as above and stored at -80°C for five years was tested in a cohort of 131 individuals. No degradation or shift in the equilibrium between ascorbate and dehydroascorbic acid was observed in either of the experiments. In conclusion, ascorbate and dehydroascorbic acid can be adequately preserved in plasma stored at -80°C following acidic deproteinization with meta-phosphoric acid containing 2 mM EDTA.

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