Ascorbate and dehydroascorbic acid as biomarkers of oxidative stress: validity of clinical data depends on vacutainer system used

Jens Lykkesfeldt

doi:10.1016/j.nutres.2011.11.005

Ascorbate and dehydroascorbic acid as biomarkers of oxidative stress: validity of clinical data depends on vacutainer system used

Jens Lykkesfeldt

Lifepharm

28 Citations (Scopus)

Abstract

Ascorbate and dehydroascorbic acid are frequently used as biomarkers of oxidative stress, but their lack of stability ex vivo and rapid postsampling interconversion continue to result in erroneous reference values. One problem is the large variety of vacutainer devices used for blood sampling purposes and the basic question of plasma vs serum as matrix. This study acquired blood samples by using 9 different and commonly used vacutainer systems followed by acidic stabilization and analysis by a well-validated method with the purpose of identifying acceptable means of collecting samples for proper ascorbate/dehydroascorbic acid analysis. In comparison, K ₃-EDTA vacutainers were superior in maintaining low ex vivo oxidation of vitamin C.

Original language	English
Journal	Nutrition Research
Volume	32
Issue number	1
Pages (from-to)	66-69
Number of pages	4
ISSN	0271-5317
DOIs	https://doi.org/10.1016/j.nutres.2011.11.005
Publication status	Published - Jan 2012

Keywords

Former LIFE faculty

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10.1016/j.nutres.2011.11.005

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title = "Ascorbate and dehydroascorbic acid as biomarkers of oxidative stress: validity of clinical data depends on vacutainer system used",

abstract = "Ascorbate and dehydroascorbic acid are frequently used as biomarkers of oxidative stress, but their lack of stability ex vivo and rapid postsampling interconversion continue to result in erroneous reference values. One problem is the large variety of vacutainer devices used for blood sampling purposes and the basic question of plasma vs serum as matrix. This study acquired blood samples by using 9 different and commonly used vacutainer systems followed by acidic stabilization and analysis by a well-validated method with the purpose of identifying acceptable means of collecting samples for proper ascorbate/dehydroascorbic acid analysis. In comparison, K 3-EDTA vacutainers were superior in maintaining low ex vivo oxidation of vitamin C.",

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journal = "Nutrition Research",

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T1 - Ascorbate and dehydroascorbic acid as biomarkers of oxidative stress

T2 - validity of clinical data depends on vacutainer system used

AU - Lykkesfeldt, Jens

PY - 2012/1

Y1 - 2012/1

N2 - Ascorbate and dehydroascorbic acid are frequently used as biomarkers of oxidative stress, but their lack of stability ex vivo and rapid postsampling interconversion continue to result in erroneous reference values. One problem is the large variety of vacutainer devices used for blood sampling purposes and the basic question of plasma vs serum as matrix. This study acquired blood samples by using 9 different and commonly used vacutainer systems followed by acidic stabilization and analysis by a well-validated method with the purpose of identifying acceptable means of collecting samples for proper ascorbate/dehydroascorbic acid analysis. In comparison, K 3-EDTA vacutainers were superior in maintaining low ex vivo oxidation of vitamin C.

AB - Ascorbate and dehydroascorbic acid are frequently used as biomarkers of oxidative stress, but their lack of stability ex vivo and rapid postsampling interconversion continue to result in erroneous reference values. One problem is the large variety of vacutainer devices used for blood sampling purposes and the basic question of plasma vs serum as matrix. This study acquired blood samples by using 9 different and commonly used vacutainer systems followed by acidic stabilization and analysis by a well-validated method with the purpose of identifying acceptable means of collecting samples for proper ascorbate/dehydroascorbic acid analysis. In comparison, K 3-EDTA vacutainers were superior in maintaining low ex vivo oxidation of vitamin C.

KW - Former LIFE faculty

KW - Ascorbate

KW - Dehydroascorbic acid

KW - Human plasma

KW - Stability

KW - Vacutainer systems ASC

KW - ascorbate

KW - DHA

KW - dehydroascorbic acid

U2 - 10.1016/j.nutres.2011.11.005

DO - 10.1016/j.nutres.2011.11.005

M3 - Journal article

C2 - 22260866

SN - 0271-5317

VL - 32

SP - 66

EP - 69

JO - Nutrition Research

JF - Nutrition Research

IS - 1

ER -

Ascorbate and dehydroascorbic acid as biomarkers of oxidative stress: validity of clinical data depends on vacutainer system used

Abstract

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