TY - JOUR
T1 - Application of thiopropyl sepharose 6B for removal of PCR inhibitors from DNA extracts of a thigh bone recovered from the sea
AU - Sørensen, Erik
AU - Hansen, Steen Holger
AU - Eriksen, Birthe
AU - Morling, Niels
N1 - Keywords: DNA; Drowning; Femur; Forensic Sciences; Humans; Male; Middle Aged; Polymerase Chain Reaction; Sepharose
PY - 2003
Y1 - 2003
N2 - PCR amplification of DNA from forensic samples often proves difficult due to the presence of inhibitors of the polymerase chain reaction. One possible way to remove PCR inhibitors from a DNA extract is the use of the affinity resin thiopropyl sepharose 6B (TS), which has been used previously for the removal of PCR inhibitors in DNA extracts originating from stains on clothing. Here we show that TS is efficient also for the removal of inhibitors from PCR extracts from a highly decomposed human thigh bone. TS treatment, however, leads to a substantial loss of DNA making the technique best suited when substantial amounts of DNA are present.
AB - PCR amplification of DNA from forensic samples often proves difficult due to the presence of inhibitors of the polymerase chain reaction. One possible way to remove PCR inhibitors from a DNA extract is the use of the affinity resin thiopropyl sepharose 6B (TS), which has been used previously for the removal of PCR inhibitors in DNA extracts originating from stains on clothing. Here we show that TS is efficient also for the removal of inhibitors from PCR extracts from a highly decomposed human thigh bone. TS treatment, however, leads to a substantial loss of DNA making the technique best suited when substantial amounts of DNA are present.
U2 - 10.1007/s00414-003-0375-6
DO - 10.1007/s00414-003-0375-6
M3 - Journal article
C2 - 12819983
SN - 0937-9827
VL - 117
SP - 245
EP - 247
JO - International Journal of Legal Medicine (Print)
JF - International Journal of Legal Medicine (Print)
IS - 4
ER -