Antioxidant synergism between ethanolic Centella asiatica extracts and α-tocopherol in model systems

TY - JOUR

T1 - Antioxidant synergism between ethanolic Centella asiatica extracts and α-tocopherol in model systems

AU - Thoo, Yin Yin

AU - Abas, Faridah

AU - Lai, Oi-Ming

AU - Ho, Chun Wai

AU - Yin, Jie

AU - Hedegaard, Rikke Susanne Vingborg

AU - Skibsted, Leif Horsfelt

AU - Tan, Chin Ping

PY - 2013/6/1

Y1 - 2013/6/1

N2 - The synergistic antioxidant effects of ethanolic extracts of Centella asiatica (CE), and α-tocopherol have been studied. The types of interactions exhibited by CE and α-tocopherol combined at different ratios were measured using three assays: 2,2′-azino-bis(3-ethylbenzothiazoline- 6-sulphonic acid) diammonium salt (ABTS) radical-scavenging capacity, the β-carotene bleaching system and liposome peroxidation assays. Fixed-fraction isobolographic analysis was used to detect any inducement of the antioxidant activity compared with the individual activities of CE and α-tocopherol. Of all synergistic combinations of CE and α-tocopherol, only fraction 2/3 showed the synergistic combination that fits well in three different assays and can be explained by the regeneration of α-tocopherol by CE despite the interaction effect of β-carotene present in the analytical assay. This phenomenon involved complex interactions between CE and α-tocopherol to exhibit different degrees of interactions that eventually increased antioxidant activity.

AB - The synergistic antioxidant effects of ethanolic extracts of Centella asiatica (CE), and α-tocopherol have been studied. The types of interactions exhibited by CE and α-tocopherol combined at different ratios were measured using three assays: 2,2′-azino-bis(3-ethylbenzothiazoline- 6-sulphonic acid) diammonium salt (ABTS) radical-scavenging capacity, the β-carotene bleaching system and liposome peroxidation assays. Fixed-fraction isobolographic analysis was used to detect any inducement of the antioxidant activity compared with the individual activities of CE and α-tocopherol. Of all synergistic combinations of CE and α-tocopherol, only fraction 2/3 showed the synergistic combination that fits well in three different assays and can be explained by the regeneration of α-tocopherol by CE despite the interaction effect of β-carotene present in the analytical assay. This phenomenon involved complex interactions between CE and α-tocopherol to exhibit different degrees of interactions that eventually increased antioxidant activity.

U2 - 10.1016/j.foodchem.2012.11.013

DO - 10.1016/j.foodchem.2012.11.013

M3 - Journal article

C2 - 23411234

SN - 0308-8146

VL - 138

SP - 1215

EP - 1219

JO - Food Chemistry

JF - Food Chemistry

IS - 2-3

ER -