Antimicrobial peptide CAP18 and its effect on Yersinia ruckeri infections in rainbow trout Oncorhynchus mykiss (Walbaum): comparing administration by injection and oral routes

Jiwan Kumar Chettri; Foojan Mehrdana; E.B Hansen; A Ebbensgaard; M.T Overgaard; A.H Lauritsen; I Dalsgaard; Kurt Buchmann

doi:10.1111/jfd.12497

Antimicrobial peptide CAP18 and its effect on Yersinia ruckeri infections in rainbow trout Oncorhynchus mykiss (Walbaum): comparing administration by injection and oral routes

Jiwan Kumar Chettri, Foojan Mehrdana, E.B Hansen, A Ebbensgaard, M.T Overgaard, A.H Lauritsen, I Dalsgaard, Kurt Buchmann

5 Citations (Scopus)

Abstract

The antimicrobial peptide CAP18 has been demonstrated to have a strong in vitro bactericidal effect on Yersinia ruckeri, but its activity in vivo has not been described. In this work, we investigated whether CAP18 protects rainbow trout Oncorhynchus mykiss (Walbaum) against enteric red mouth disease caused by this pathogen either following i.p. injection or by oral administration (in feed). It was found that injection of CAP18 into juvenile rainbow trout before exposure to Y. ruckeri was associated with lowered mortality compared to non-medicated fish although it was less effective than the conventional antibiotic oxolinic acid. Oral administration of CAP18 to trout did not prevent infection. The proteolytic effect of secretions on the peptide CAP18 in the fish gastrointestinal tract is suggested to account for the inferior effect of oral administration.

Original language	English
Journal	Journal of Fish Diseases
Volume	40
Issue number	1
Pages (from-to)	97-104
ISSN	0140-7775
DOIs	https://doi.org/10.1111/jfd.12497
Publication status	Published - 1 Jan 2017

Access to Document

10.1111/jfd.12497

Cite this

Chettri, J. K., Mehrdana, F., Hansen, E. B., Ebbensgaard, A., Overgaard, M. T., Lauritsen, A. H., Dalsgaard, I., & Buchmann, K. (2017). Antimicrobial peptide CAP18 and its effect on Yersinia ruckeri infections in rainbow trout Oncorhynchus mykiss (Walbaum): comparing administration by injection and oral routes. Journal of Fish Diseases, 40(1), 97-104. https://doi.org/10.1111/jfd.12497

Antimicrobial peptide CAP18 and its effect on Yersinia ruckeri infections in rainbow trout Oncorhynchus mykiss (Walbaum): comparing administration by injection and oral routes. / Chettri, Jiwan Kumar; Mehrdana, Foojan; Hansen, E.B et al.
In: Journal of Fish Diseases, Vol. 40, No. 1, 01.01.2017, p. 97-104.

Research output: Contribution to journal › Journal article › Research › peer-review

Chettri, JK, Mehrdana, F, Hansen, EB, Ebbensgaard, A, Overgaard, MT, Lauritsen, AH, Dalsgaard, I & Buchmann, K 2017, 'Antimicrobial peptide CAP18 and its effect on Yersinia ruckeri infections in rainbow trout Oncorhynchus mykiss (Walbaum): comparing administration by injection and oral routes', Journal of Fish Diseases, vol. 40, no. 1, pp. 97-104. https://doi.org/10.1111/jfd.12497

@article{c2a901fdbccf41e7ab0b5564e293d599,

title = "Antimicrobial peptide CAP18 and its effect on Yersinia ruckeri infections in rainbow trout Oncorhynchus mykiss (Walbaum): comparing administration by injection and oral routes",

abstract = "The antimicrobial peptide CAP18 has been demonstrated to have a strong in vitro bactericidal effect on Yersinia ruckeri, but its activity in vivo has not been described. In this work, we investigated whether CAP18 protects rainbow trout Oncorhynchus mykiss (Walbaum) against enteric red mouth disease caused by this pathogen either following i.p. injection or by oral administration (in feed). It was found that injection of CAP18 into juvenile rainbow trout before exposure to Y. ruckeri was associated with lowered mortality compared to non-medicated fish although it was less effective than the conventional antibiotic oxolinic acid. Oral administration of CAP18 to trout did not prevent infection. The proteolytic effect of secretions on the peptide CAP18 in the fish gastrointestinal tract is suggested to account for the inferior effect of oral administration.",

author = "Chettri, {Jiwan Kumar} and Foojan Mehrdana and E.B Hansen and A Ebbensgaard and M.T Overgaard and A.H Lauritsen and I Dalsgaard and Kurt Buchmann",

year = "2017",

month = jan,

day = "1",

doi = "10.1111/jfd.12497",

language = "English",

volume = "40",

pages = "97--104",

journal = "Journal of Fish Diseases",

issn = "0140-7775",

publisher = "Wiley-Blackwell",

number = "1",

}

TY - JOUR

T1 - Antimicrobial peptide CAP18 and its effect on Yersinia ruckeri infections in rainbow trout Oncorhynchus mykiss (Walbaum): comparing administration by injection and oral routes

AU - Chettri, Jiwan Kumar

AU - Mehrdana, Foojan

AU - Hansen, E.B

AU - Ebbensgaard, A

AU - Overgaard, M.T

AU - Lauritsen, A.H

AU - Dalsgaard, I

AU - Buchmann, Kurt

PY - 2017/1/1

Y1 - 2017/1/1

N2 - The antimicrobial peptide CAP18 has been demonstrated to have a strong in vitro bactericidal effect on Yersinia ruckeri, but its activity in vivo has not been described. In this work, we investigated whether CAP18 protects rainbow trout Oncorhynchus mykiss (Walbaum) against enteric red mouth disease caused by this pathogen either following i.p. injection or by oral administration (in feed). It was found that injection of CAP18 into juvenile rainbow trout before exposure to Y. ruckeri was associated with lowered mortality compared to non-medicated fish although it was less effective than the conventional antibiotic oxolinic acid. Oral administration of CAP18 to trout did not prevent infection. The proteolytic effect of secretions on the peptide CAP18 in the fish gastrointestinal tract is suggested to account for the inferior effect of oral administration.

AB - The antimicrobial peptide CAP18 has been demonstrated to have a strong in vitro bactericidal effect on Yersinia ruckeri, but its activity in vivo has not been described. In this work, we investigated whether CAP18 protects rainbow trout Oncorhynchus mykiss (Walbaum) against enteric red mouth disease caused by this pathogen either following i.p. injection or by oral administration (in feed). It was found that injection of CAP18 into juvenile rainbow trout before exposure to Y. ruckeri was associated with lowered mortality compared to non-medicated fish although it was less effective than the conventional antibiotic oxolinic acid. Oral administration of CAP18 to trout did not prevent infection. The proteolytic effect of secretions on the peptide CAP18 in the fish gastrointestinal tract is suggested to account for the inferior effect of oral administration.

U2 - 10.1111/jfd.12497

DO - 10.1111/jfd.12497

M3 - Journal article

C2 - 27334068

SN - 0140-7775

VL - 40

SP - 97

EP - 104

JO - Journal of Fish Diseases

JF - Journal of Fish Diseases

IS - 1

ER -

Antimicrobial peptide CAP18 and its effect on Yersinia ruckeri infections in rainbow trout Oncorhynchus mykiss (Walbaum): comparing administration by injection and oral routes

Abstract

Access to Document

Fingerprint

Cite this