Annexin A7 is required for ESCRT III-mediated plasma membrane repair

Stine Lauritzen Sønder; Theresa Louise Boye; Regine Tölle; Jörn Dengjel; Kenji Maeda; Marja Jäättelä; Adam Cohen Simonsen; Jyoti K. Jaiswal; Jesper Nylandsted

doi:10.1038/s41598-019-43143-4

Annexin A7 is required for ESCRT III-mediated plasma membrane repair

Stine Lauritzen Sønder, Theresa Louise Boye, Regine Tölle, Jörn Dengjel, Kenji Maeda, Marja Jäättelä, Adam Cohen Simonsen, Jyoti K. Jaiswal, Jesper Nylandsted^*

^*Corresponding author for this work

Morphogenesis and Differentiation Program

21 Citations (Scopus)

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Abstract

The plasma membrane of eukaryotic cells forms the essential barrier to the extracellular environment, and thus plasma membrane disruptions pose a fatal threat to cells. Here, using invasive breast cancer cells we show that the Ca ²⁺ - and phospholipid-binding protein annexin A7 is part of the plasma membrane repair response by enabling assembly of the endosomal sorting complex required for transport (ESCRT) III. Following injury to the plasma membrane and Ca ²⁺ flux into the cytoplasm, annexin A7 forms a complex with apoptosis linked gene-2 (ALG-2) to facilitate proper recruitment and binding of ALG-2 and ALG-2-interacting protein X (ALIX) to the damaged membrane. ALG-2 and ALIX assemble the ESCRT III complex, which helps excise and shed the damaged portion of the plasma membrane during wound healing. Our results reveal a novel function of annexin A7 – enabling plasma membrane repair by regulating ESCRT III-mediated shedding of injured plasma membrane.

Original language	English
Article number	6726
Journal	Scientific Reports
Volume	9
Issue number	1
Number of pages	12
ISSN	2045-2322
DOIs	https://doi.org/10.1038/s41598-019-43143-4
Publication status	Published - 2019

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Annexin A7 is required for ESCRT III-mediated plasma membrane repairFinal published version, 2.37 MBLicence: CC BY

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title = "Annexin A7 is required for ESCRT III-mediated plasma membrane repair",

abstract = " The plasma membrane of eukaryotic cells forms the essential barrier to the extracellular environment, and thus plasma membrane disruptions pose a fatal threat to cells. Here, using invasive breast cancer cells we show that the Ca 2+ - and phospholipid-binding protein annexin A7 is part of the plasma membrane repair response by enabling assembly of the endosomal sorting complex required for transport (ESCRT) III. Following injury to the plasma membrane and Ca 2+ flux into the cytoplasm, annexin A7 forms a complex with apoptosis linked gene-2 (ALG-2) to facilitate proper recruitment and binding of ALG-2 and ALG-2-interacting protein X (ALIX) to the damaged membrane. ALG-2 and ALIX assemble the ESCRT III complex, which helps excise and shed the damaged portion of the plasma membrane during wound healing. Our results reveal a novel function of annexin A7 – enabling plasma membrane repair by regulating ESCRT III-mediated shedding of injured plasma membrane. ",

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AU - Sønder, Stine Lauritzen

AU - Boye, Theresa Louise

AU - Tölle, Regine

AU - Dengjel, Jörn

AU - Maeda, Kenji

AU - Jäättelä, Marja

AU - Simonsen, Adam Cohen

AU - Jaiswal, Jyoti K.

AU - Nylandsted, Jesper

PY - 2019

Y1 - 2019

N2 - The plasma membrane of eukaryotic cells forms the essential barrier to the extracellular environment, and thus plasma membrane disruptions pose a fatal threat to cells. Here, using invasive breast cancer cells we show that the Ca 2+ - and phospholipid-binding protein annexin A7 is part of the plasma membrane repair response by enabling assembly of the endosomal sorting complex required for transport (ESCRT) III. Following injury to the plasma membrane and Ca 2+ flux into the cytoplasm, annexin A7 forms a complex with apoptosis linked gene-2 (ALG-2) to facilitate proper recruitment and binding of ALG-2 and ALG-2-interacting protein X (ALIX) to the damaged membrane. ALG-2 and ALIX assemble the ESCRT III complex, which helps excise and shed the damaged portion of the plasma membrane during wound healing. Our results reveal a novel function of annexin A7 – enabling plasma membrane repair by regulating ESCRT III-mediated shedding of injured plasma membrane.

AB - The plasma membrane of eukaryotic cells forms the essential barrier to the extracellular environment, and thus plasma membrane disruptions pose a fatal threat to cells. Here, using invasive breast cancer cells we show that the Ca 2+ - and phospholipid-binding protein annexin A7 is part of the plasma membrane repair response by enabling assembly of the endosomal sorting complex required for transport (ESCRT) III. Following injury to the plasma membrane and Ca 2+ flux into the cytoplasm, annexin A7 forms a complex with apoptosis linked gene-2 (ALG-2) to facilitate proper recruitment and binding of ALG-2 and ALG-2-interacting protein X (ALIX) to the damaged membrane. ALG-2 and ALIX assemble the ESCRT III complex, which helps excise and shed the damaged portion of the plasma membrane during wound healing. Our results reveal a novel function of annexin A7 – enabling plasma membrane repair by regulating ESCRT III-mediated shedding of injured plasma membrane.

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DO - 10.1038/s41598-019-43143-4

M3 - Journal article

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AN - SCOPUS:85064905404

SN - 2045-2322

VL - 9

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 6726

ER -

Annexin A7 is required for ESCRT III-mediated plasma membrane repair

Abstract

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