Abstract
AIM: The current study was designed to characterize the anticancer effects of clotrimazole on human cutaneous melanoma cells.
MATERIALS AND METHODS: The v-raf murine sarcoma viral oncogene homolog B1 V600E mutant melanoma cell line A375 was used as an in vitro model. Characterization tools included analyses of cell viability, gene expression, cell-cycle progression, annexin V reactivity and internucleosomal DNA fragmentation.
RESULTS: Clotrimazole induced cytotoxicity in A375 human melanoma cells without significant changes of human keratinocyte cell viability. Clotrimazole, at a concentration that approximates the inhibitory concentration 50% (IC50) value (i.e. 10 μM), reduced the expression of hexokinase type-II, induced cell-cycle arrest at G1-S phase transition, altered annexin V reactivity and induced DNA fragmentation without evidence of necrosis.
CONCLUSION: The current study provides evidence of a remarkable pro-apoptotic effect by clotrimazole against human melanoma cells, with a different mechanism of action and timeline of the apoptosis-related events when compared to cisplatin.
| Original language | English |
|---|---|
| Journal | Anticancer Research |
| Volume | 35 |
| Issue number | 7 |
| Pages (from-to) | 3781-6 |
| Number of pages | 6 |
| ISSN | 0250-7005 |
| Publication status | Published - 1 Jul 2015 |
Keywords
- Annexin A5
- Antineoplastic Agents
- Apoptosis
- Cell Line, Tumor
- Cell Survival
- Cisplatin
- Clotrimazole
- DNA Fragmentation
- G1 Phase Cell Cycle Checkpoints
- Humans
- Keratinocytes
- Melanoma