Analysis of enteroendocrine cell populations in the human colon

Patricia Martins; Josiane Fakhry; Enio Chaves de Oliveira; Billie Hunne; Linda J Fothergill; Mitchell Ringuet; Débora d'Ávila Reis; Jens F Rehfeld; Brid Callaghan; John B Furness

doi:10.1007/s00441-016-2530-7

Analysis of enteroendocrine cell populations in the human colon

Patricia Martins, Josiane Fakhry, Enio Chaves de Oliveira, Billie Hunne, Linda J Fothergill, Mitchell Ringuet, Débora d'Ávila Reis, Jens F Rehfeld, Brid Callaghan, John B Furness

Department of Clinical Medicine

16 Citations (Scopus)

Abstract

Recent studies have shown that patterns of colocalisation of hormones in enteroendocrine cells are more complex than previously appreciated and that the patterns differ substantially between species. In this study, the human sigmoid colon is investigated by immunohistochemistry for the presence of gastrointestinal hormones and their colocalisation. The segments of colon were distant from the pathology that led to colectomy and appeared structurally normal. Only four hormones, 5-hydroxytryptamine (5-HT), glucagon-like peptide 1 (GLP-1), peptide YY (PYY) and somatostatin, were common in enteroendocrine cells of the human colon. Cholecystokinin, present in the colon of some species, was absent, as were glucose-dependent insulinotropic peptide, ghrelin and motilin. Neurotensin cells were extremely rare. The most numerous cells were 5-HT cells, some of which also contained PYY or somatostatin and very rarely GLP-1. Almost all GLP-1 cells contained PYY. It is concluded that enteroendocrine cells of the human colon, like those of other regions and species, exhibit overlapping patterns of hormone colocalisation and that the hormones and their patterns of expression differ between human and other species.

Original language	English
Journal	Cell and Tissue Research
Volume	367
Issue number	2
Pages (from-to)	161-168
ISSN	0302-766X
DOIs	https://doi.org/10.1007/s00441-016-2530-7
Publication status	Published - 1 Feb 2017

Keywords

Cell Count
Colon/cytology
Enteroendocrine Cells/cytology
Hormones/metabolism
Humans
Jejunum/cytology
Staining and Labeling

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10.1007/s00441-016-2530-7

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title = "Analysis of enteroendocrine cell populations in the human colon",

abstract = "Recent studies have shown that patterns of colocalisation of hormones in enteroendocrine cells are more complex than previously appreciated and that the patterns differ substantially between species. In this study, the human sigmoid colon is investigated by immunohistochemistry for the presence of gastrointestinal hormones and their colocalisation. The segments of colon were distant from the pathology that led to colectomy and appeared structurally normal. Only four hormones, 5-hydroxytryptamine (5-HT), glucagon-like peptide 1 (GLP-1), peptide YY (PYY) and somatostatin, were common in enteroendocrine cells of the human colon. Cholecystokinin, present in the colon of some species, was absent, as were glucose-dependent insulinotropic peptide, ghrelin and motilin. Neurotensin cells were extremely rare. The most numerous cells were 5-HT cells, some of which also contained PYY or somatostatin and very rarely GLP-1. Almost all GLP-1 cells contained PYY. It is concluded that enteroendocrine cells of the human colon, like those of other regions and species, exhibit overlapping patterns of hormone colocalisation and that the hormones and their patterns of expression differ between human and other species.",

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AU - Martins, Patricia

AU - Fakhry, Josiane

AU - de Oliveira, Enio Chaves

AU - Hunne, Billie

AU - Fothergill, Linda J

AU - Ringuet, Mitchell

AU - Reis, Débora d'Ávila

AU - Rehfeld, Jens F

AU - Callaghan, Brid

AU - Furness, John B

PY - 2017/2/1

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N2 - Recent studies have shown that patterns of colocalisation of hormones in enteroendocrine cells are more complex than previously appreciated and that the patterns differ substantially between species. In this study, the human sigmoid colon is investigated by immunohistochemistry for the presence of gastrointestinal hormones and their colocalisation. The segments of colon were distant from the pathology that led to colectomy and appeared structurally normal. Only four hormones, 5-hydroxytryptamine (5-HT), glucagon-like peptide 1 (GLP-1), peptide YY (PYY) and somatostatin, were common in enteroendocrine cells of the human colon. Cholecystokinin, present in the colon of some species, was absent, as were glucose-dependent insulinotropic peptide, ghrelin and motilin. Neurotensin cells were extremely rare. The most numerous cells were 5-HT cells, some of which also contained PYY or somatostatin and very rarely GLP-1. Almost all GLP-1 cells contained PYY. It is concluded that enteroendocrine cells of the human colon, like those of other regions and species, exhibit overlapping patterns of hormone colocalisation and that the hormones and their patterns of expression differ between human and other species.

AB - Recent studies have shown that patterns of colocalisation of hormones in enteroendocrine cells are more complex than previously appreciated and that the patterns differ substantially between species. In this study, the human sigmoid colon is investigated by immunohistochemistry for the presence of gastrointestinal hormones and their colocalisation. The segments of colon were distant from the pathology that led to colectomy and appeared structurally normal. Only four hormones, 5-hydroxytryptamine (5-HT), glucagon-like peptide 1 (GLP-1), peptide YY (PYY) and somatostatin, were common in enteroendocrine cells of the human colon. Cholecystokinin, present in the colon of some species, was absent, as were glucose-dependent insulinotropic peptide, ghrelin and motilin. Neurotensin cells were extremely rare. The most numerous cells were 5-HT cells, some of which also contained PYY or somatostatin and very rarely GLP-1. Almost all GLP-1 cells contained PYY. It is concluded that enteroendocrine cells of the human colon, like those of other regions and species, exhibit overlapping patterns of hormone colocalisation and that the hormones and their patterns of expression differ between human and other species.

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KW - Enteroendocrine Cells/cytology

KW - Hormones/metabolism

KW - Humans

KW - Jejunum/cytology

KW - Staining and Labeling

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DO - 10.1007/s00441-016-2530-7

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SP - 161

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JO - Cell and Tissue Research

JF - Cell and Tissue Research

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ER -

Analysis of enteroendocrine cell populations in the human colon

Abstract

Keywords

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