Analysis of enteroendocrine cell populations in the human colon

Patricia Martins; Josiane Fakhry; Enio Chaves de Oliveira; Billie Hunne; Linda J Fothergill; Mitchell Ringuet; Débora d'Ávila Reis; Jens F Rehfeld; Brid Callaghan; John B Furness

doi:10.1007/s00441-016-2530-7

Analysis of enteroendocrine cell populations in the human colon

Patricia Martins, Josiane Fakhry, Enio Chaves de Oliveira, Billie Hunne, Linda J Fothergill, Mitchell Ringuet, Débora d'Ávila Reis, Jens F Rehfeld, Brid Callaghan, John B Furness

Institut for Klinisk Medicin

16 Citationer (Scopus)

Abstract

Recent studies have shown that patterns of colocalisation of hormones in enteroendocrine cells are more complex than previously appreciated and that the patterns differ substantially between species. In this study, the human sigmoid colon is investigated by immunohistochemistry for the presence of gastrointestinal hormones and their colocalisation. The segments of colon were distant from the pathology that led to colectomy and appeared structurally normal. Only four hormones, 5-hydroxytryptamine (5-HT), glucagon-like peptide 1 (GLP-1), peptide YY (PYY) and somatostatin, were common in enteroendocrine cells of the human colon. Cholecystokinin, present in the colon of some species, was absent, as were glucose-dependent insulinotropic peptide, ghrelin and motilin. Neurotensin cells were extremely rare. The most numerous cells were 5-HT cells, some of which also contained PYY or somatostatin and very rarely GLP-1. Almost all GLP-1 cells contained PYY. It is concluded that enteroendocrine cells of the human colon, like those of other regions and species, exhibit overlapping patterns of hormone colocalisation and that the hormones and their patterns of expression differ between human and other species.

Originalsprog	Engelsk
Tidsskrift	Cell and Tissue Research
Vol/bind	367
Udgave nummer	2
Sider (fra-til)	161-168
ISSN	0302-766X
DOI	https://doi.org/10.1007/s00441-016-2530-7
Status	Udgivet - 1 feb. 2017

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10.1007/s00441-016-2530-7

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abstract = "Recent studies have shown that patterns of colocalisation of hormones in enteroendocrine cells are more complex than previously appreciated and that the patterns differ substantially between species. In this study, the human sigmoid colon is investigated by immunohistochemistry for the presence of gastrointestinal hormones and their colocalisation. The segments of colon were distant from the pathology that led to colectomy and appeared structurally normal. Only four hormones, 5-hydroxytryptamine (5-HT), glucagon-like peptide 1 (GLP-1), peptide YY (PYY) and somatostatin, were common in enteroendocrine cells of the human colon. Cholecystokinin, present in the colon of some species, was absent, as were glucose-dependent insulinotropic peptide, ghrelin and motilin. Neurotensin cells were extremely rare. The most numerous cells were 5-HT cells, some of which also contained PYY or somatostatin and very rarely GLP-1. Almost all GLP-1 cells contained PYY. It is concluded that enteroendocrine cells of the human colon, like those of other regions and species, exhibit overlapping patterns of hormone colocalisation and that the hormones and their patterns of expression differ between human and other species.",

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T1 - Analysis of enteroendocrine cell populations in the human colon

AU - Martins, Patricia

AU - Fakhry, Josiane

AU - de Oliveira, Enio Chaves

AU - Hunne, Billie

AU - Fothergill, Linda J

AU - Ringuet, Mitchell

AU - Reis, Débora d'Ávila

AU - Rehfeld, Jens F

AU - Callaghan, Brid

AU - Furness, John B

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N2 - Recent studies have shown that patterns of colocalisation of hormones in enteroendocrine cells are more complex than previously appreciated and that the patterns differ substantially between species. In this study, the human sigmoid colon is investigated by immunohistochemistry for the presence of gastrointestinal hormones and their colocalisation. The segments of colon were distant from the pathology that led to colectomy and appeared structurally normal. Only four hormones, 5-hydroxytryptamine (5-HT), glucagon-like peptide 1 (GLP-1), peptide YY (PYY) and somatostatin, were common in enteroendocrine cells of the human colon. Cholecystokinin, present in the colon of some species, was absent, as were glucose-dependent insulinotropic peptide, ghrelin and motilin. Neurotensin cells were extremely rare. The most numerous cells were 5-HT cells, some of which also contained PYY or somatostatin and very rarely GLP-1. Almost all GLP-1 cells contained PYY. It is concluded that enteroendocrine cells of the human colon, like those of other regions and species, exhibit overlapping patterns of hormone colocalisation and that the hormones and their patterns of expression differ between human and other species.

AB - Recent studies have shown that patterns of colocalisation of hormones in enteroendocrine cells are more complex than previously appreciated and that the patterns differ substantially between species. In this study, the human sigmoid colon is investigated by immunohistochemistry for the presence of gastrointestinal hormones and their colocalisation. The segments of colon were distant from the pathology that led to colectomy and appeared structurally normal. Only four hormones, 5-hydroxytryptamine (5-HT), glucagon-like peptide 1 (GLP-1), peptide YY (PYY) and somatostatin, were common in enteroendocrine cells of the human colon. Cholecystokinin, present in the colon of some species, was absent, as were glucose-dependent insulinotropic peptide, ghrelin and motilin. Neurotensin cells were extremely rare. The most numerous cells were 5-HT cells, some of which also contained PYY or somatostatin and very rarely GLP-1. Almost all GLP-1 cells contained PYY. It is concluded that enteroendocrine cells of the human colon, like those of other regions and species, exhibit overlapping patterns of hormone colocalisation and that the hormones and their patterns of expression differ between human and other species.

KW - Cell Count

KW - Colon/cytology

KW - Enteroendocrine Cells/cytology

KW - Hormones/metabolism

KW - Humans

KW - Jejunum/cytology

KW - Staining and Labeling

U2 - 10.1007/s00441-016-2530-7

DO - 10.1007/s00441-016-2530-7

M3 - Journal article

C2 - 27844204

SN - 0302-766X

VL - 367

SP - 161

EP - 168

JO - Cell and Tissue Research

JF - Cell and Tissue Research

IS - 2

ER -

Analysis of enteroendocrine cell populations in the human colon

Abstract

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